Citation: LI Lili, CHEN Bingnian, DENG Yangyang, XIE Lefang, XING Rui, WANG Li. Inhibitory Effects of Dawson Type Polyoxometalates on Tyrosinase[J]. Chinese Journal of Applied Chemistry, ;2017, 34(1): 83-89. doi: 10.11944/j.issn.1000-0518.2017.01.160094 shu

Inhibitory Effects of Dawson Type Polyoxometalates on Tyrosinase

LI Lili ^a ,
CHEN Bingnian ^b ,
DENG Yangyang ^a ,
XIE Lefang ^a ,
XING Rui ^a ,
WANG Li ^a,*,,

a.
College of Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China
b.
Yanwu Affiliated Hospital of Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361005, China

Corresponding author: WANG Li, wanglimerry@jmu.edu.cn
Received Date: 7 March 2016
Revised Date: 25 April 2016
Accepted Date: 25 April 2016

Fund Project: the National Natural Science Foundation of China 21371072

Figures(5)

Tyrosinase is a complicated copper containing oxidoreductase that is common in microorganism, plants, animals and human body, which plays a crucial role in melanin biosynthesis. Currently researches about tyrosinase inhibitors are mostly focused on natural extracts and organism. However, inorganic Dawson type polyoxometalates as tyrosinase inhibitors have been less reported. Two kinds of Dawson type polyoxometalates were synthesized and characterized by ultraviolet spectroscopy and infrared spectral analysis. The inhibitory effects of H₆[P₂Mo₁₈O₆₂] and H₈[P₂Mo₁₇Cr (OH₂) O₆₁](abbreviated to P₂Mo₁₈ and P₂Mo₁₇Cr, respectively) on mushroom tyrosinase were investigated by ultraviolet spectrophotometry and enzymatic kinetics methods. P₂Mo₁₈ and P₂Mo₁₇Cr have significant inhibitory effects on tyrosinase, and the IC₅₀ values of P₂Mo₁₈ and P₂Mo₁₇Cr are (0.482±0.009) mmol/L and (0.503±0.011) mmol/L for diphenolase, respectively. Enzymatic kinetics analysis indicates that P₂Mo₁₈ and P₂Mo₁₇Cr are reversible and competitive inhibitor, and the inhibition constant of P₂Mo₁₈ and P₂Mo₁₇Cr are 0.212 mmol/L and 0.249 mmol/L, respectively. Considering of IC₅₀ values and inhibition constants, the inhibitory effect of P₂Mo₁₈ is slightly better than that of P₂Mo₁₇Cr. In conclusion, P₂Mo₁₈ and P₂Mo₁₇Cr show effective antityrosinase activities. Compared with arbutin, P₂Mo₁₈ and P₂Mo₁₇Cr have much more inhibitory effects on the diphenolase activity of tyrosinase. This study may provide reference foundation for the further study of the tyrosinase inhibitors, and also offer useful information for the comprehensive utilization of polyoxometalates.
- tyrosinase,
- inhibitor,
- polyoxometalates,
- diphenolase activity
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