Citation: ZHANG Tieli, CHAI Guoping, HE Caixia, WANG Lei. Preparation of p-Hydroxybenzoic Acid-Imprinted Polymer Particulates and Application to Solid Phase Extraction[J]. Chinese Journal of Applied Chemistry, ;2016, 33(2): 229-237. doi: 10.11944/j.issn.1000-0518.2016.02.150184 shu

Preparation of p-Hydroxybenzoic Acid-Imprinted Polymer Particulates and Application to Solid Phase Extraction

ZHANG Tieli ^a,, ,
CHAI Guoping ^a ,
HE Caixia ^a,b ,
WANG Lei ^a

1.
a Department of Chemistry, Tangshan Nomoral College, Tangshan, Hebei 063000, China;
2.
b College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China

Corresponding author: ZHANG Tieli,
Received Date: 2 June 2015
Available Online: 29 October 2015
Fund Project:

p-Hydroxybenzoic acid(p-HBA)-imprinted polymer particulates were prepared using p-HBA as the template, acrylamide(AM) as the functional monomer and ethylene glycol dimethacrylate as the crosslinker in three different amounts of p-HBA and under two different concentrations of pre-polymerization mixtures via precipitation polymerization. Their rebinding performances for p-HBA were characterized by high-performance liquid chromatography. The results reveal that both the amounts of p-HBA and the concentrations of pre-polymerization mixtures exhibit notable influence on the binding performances of p-HBA-imprinted polymers. The p-HBA-imprinted polymer(P₂) with high affinity(k'=4.01) and selectivity for p-HBA is obtained when the amount of p-HBA is 1.0 mmol, which is corresponding to the molar ratio of 1:2(p-HBA/AM), in 37.5 mL of acetonitrile solvent. In addition, the imprinted polymer P₂ was chosen as the adsorbent to make the molecularly imprinted solid phase extraction(MISPE) column and the MISPE method for the extraction of p-HBA was developed. The MISPE column capacities for p-HBA are 6.91 μg/100 mg and 1.93 μg/100 mg, respectively, which are determined under two different modes of loading samples. The rhizoma gastrodiae samples spiked with three different amounts of p-HBA are extracted and the recoveries of p-HBA range from 76.8% to 86.6%, with the RSD of 3.4%~6.2%(n=3). The results demonstrate that the p-HBA-imprinted polymer particulates prepared using AM as the functional monomer by precipitation polymerization are suitable as the adsorbent of solid phase extraction column for the selective separation and purification of p-HBA in the real sample of rhizoma gastrodiae.
- p-hydroxybenzoic acid,
- precipitation polymerization,
- molecular recognition,
- molecularly imprinted solid phase extraction
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