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Citation: YANG Shaoming, CHEN Yansheng, LI Ruiqin, LONG Qiyang, DING Suyou. One-step Preparation of Horseradish Peroxidase Biosensor via Electrodeposition[J]. Chinese Journal of Applied Chemistry, ;2015, 32(7): 849-854. doi: 10.11944/j.issn.1000-0518.2015.07.140401 shu

One-step Preparation of Horseradish Peroxidase Biosensor via Electrodeposition

1.
Department of Chemistry and Chemical Engineering, School of Sciences, East China Jiaotong University, Nanchang 330013, China

Corresponding author: YANG Shaoming,
Received Date: 19 November 2014
Available Online: 23 December 2014
Fund Project: 国家自然科学基金资助项目(21465012,21065004) (21465012,21065004)

Horseradish peroxidase (HRP) and carbon nanotubes (CNTs) were co-immobilized on the base electrode surface during the process of potentiostatic electrodeposition of cobalt-aluminium layered double hydroxide (Co Al-LDH).The as-prepared Co Al-LDH-HRP-CNTs modified electrode was used for the determination of hydrogen peroxide.The morphology of Co Al-LDH-HRP-CNTs was characterized by SEM.The preparation process of the modified electrode was monitored by the electrochemical spectroscopy.The electrochemical behavior of the modified electrode was studied by cyclic voltammetry.The effect of p H and work potential on the catalytic reduction performance of the modified electrode was investigated.The biosensor shows a good linear range of 2.5×10^-6~3.35×10^-4mol/L for the determination of hydrogen peroxide with the sensitivity of 0.0049 A·L/mol.
- cobalt-aluminium layered double hydroxide,
- carbon nanotubes,
- horseradish peroxidase,
- electrodeposition,
- biosensor
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