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Citation: ZHANG Mei, YAO Bao-Jin, YANG Yong-Gang, HU Yao-Zhong, LI Yin-Qing, WANG Qun, ZHAO Yu. Analysis of Protein Expression Profiles of Sika Deer Antler at Nascent and Rapid Growth Stages[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(11): 1732-1738. doi: 10.11895/j.issn.0253-3820.181463 shu

Analysis of Protein Expression Profiles of Sika Deer Antler at Nascent and Rapid Growth Stages

¹ Innovation Practice Center;
² Chinese Medicine and Bioengineering Research and Development Center;
³ The Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130117, China

Received Date: 16 July 2018
Revised Date: 21 August 2018

Fund Project: This work was supported by the Science and Technology Project of Jilin Provincial Education Department (Nos. JJKH20170721KJ, JJKH20181272KJ, JJKH20181269KJ), the Science and Technology Development Project of Jilin Province (No. 20170520044JH), the TCM Clinical Research Center for Bone Diseases of Jilin Province (No. 20180623048TC) and the Transformation of Scientific and Technological Achievement Project of Jilin Province (No. 201603072YY).

The protein expression profiles of the northeast Chinese Sika deer antler at nascent stage (15 d) and rapid growth stage (60 d) were investigated by using the isotope labeled protein relative and absolute quantification (iTRAQ), liquid chromatography-mass spectrometry and bioinformatics analysis. A total of 127 kinds of differentially expressed proteins were identified. Compared to rapid growth stage, there were 49 kinds of differentially expressed proteins significantly up-regulated at rapid growth stage, and there were 78 kinds of differentially expressed proteins significantly down-regulated. These differentially expressed proteins were mainly extracellular matrix components and involved in the regulation of nerve growth and blood circulation process. Among them, a variety of extracellular matrix proteins were synthesized at nascent stage, however, the expression of these proteins was significantly decreased at rapid growth stage. Meanwhile, the expression of functional proteins involved in the regulation of nerve growth and blood circulation was significantly up-regulated. These differentially expressed proteins coordinated to regulate the growth rate of Sika deer antler. The results provide the basic data for further revealing the growth mechanism, and important guiding for the clinical application.
- Sika deer antler,
- Nascent stage,
- Rapid growth stage,
- Protein expression profile,
- Differential expression analysis
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