Citation: ZHANG Miao-Miao, GUO Xiao-Peng, LIU Rui-Yuan, MA Liang, GAO Yue, LU Dong, LI Wen-Jian. Lipidomics Studies on Mitochondrial Damage of Saccharomyces Cerevisiae Induced by Heavy Ion Beam Radiation[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(11): 1714-1723. doi: 10.11895/j.issn.0253-3820.181378 shu

Lipidomics Studies on Mitochondrial Damage of Saccharomyces Cerevisiae Induced by Heavy Ion Beam Radiation

ZHANG Miao-Miao ^1,2,3, ,
GUO Xiao-Peng ^1,2 ,
LIU Rui-Yuan ¹ ,
MA Liang ^1,3 ,
GAO Yue ^1,2 ,
LU Dong ^1,3 ,
LI Wen-Jian ^1,3

¹ Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 73000, China;
² University of Chinese Academy of Sciences, Beijing 100049, China;
³ Gansu Provincial Key Laboratory of Microbial Resources Exploition and Application, Lanzhou 73000, China

Received Date: 5 June 2018
Revised Date: 8 August 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 11575259) and the Key Deployment Project of Chinese Academy of Sciences (No.KFZD-SW-109)

Ultra high performance liquid chromatography-electrospray ionization-mass spectrometry (UHPLC-ESI-MS) combined with multivariate and univariate data analysis methods was used to analyze lipid metabolomics of mitochondria-damaged Saccharomyces cerevisiae (ResD-4) after heavy ion beam radiation. The composition of lipid metabolites in control and ResD-4 was detected, and a total of 648 lipid molecules were identified. The results showed that the orthogonal partial least squares discriminant analysis (OPLS-DA) could accurately distinguish the lipid metabolites of ResD-4 and control. Based on variable importance for the projection (VIP), variation multiple (FC) and difference analysis (p), 12 subfamilies, 54 types of significant difference in lipid molecules were finally screened including diacylglycerol (DG)(16:0/18:1), lysophosphatidylcholine (LPC) (18:1), lysophosphatidylethanolamine (LPE) (16:0), lysophosphatidylinositol (LPI) (18:1), lysophosphatidylserine (LPS) (18:1), phosphatidic acid (PA) (16:0/18:1), phosphatidylcholine (PC) (31:1), phosphatidylethanolamine (PE) (16:0/10:0), phosphatidylinositol (PI) (15:0/18:1), phosphatidylserine (PS) (16:0/16:1), sulfoquinovosyl diacylglycerol (SQDG) (37:4), triglyceride (TG) (15:0/16:0/18:1), etc. Significant differences in the expression level of lipid molecules showed that the expression levels of DG, TG, SQDG, PA, PC, PI, LPC, LPE and LPI were up-regulated, and PE, PS and LPS were down-regulated. The results showed that S. cerevisiae might respond by adjusting the content of lipid components after mitochondrial damage caused by heavy ion beam radiation. The lipid molecules with significant difference expression level may be potential markers for S. cerevisiae mitochondrial damage induced by heavy ion beam radiation.
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