Citation: ZHENG Yan, LIU Shu, SONG Feng-Rui, LIU Zhi-Qiang. Study on Compatibility Mechanism of Ding-Zhi-Xiao-Wan Formula in Treating Alzheimer's Disease Based on Aβ_25-35 Induced PC12 Cells[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(11): 1724-1731. doi: 10.11895/j.issn.0253-3820.181318 shu

Study on Compatibility Mechanism of Ding-Zhi-Xiao-Wan Formula in Treating Alzheimer's Disease Based on Aβ_25-35 Induced PC12 Cells

ZHENG Yan ^1,2, ,
LIU Shu ¹ ,
SONG Feng-Rui ¹ ,
LIU Zhi-Qiang ^1,3

¹ National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry & Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
² University of Chinese Academy of Sciences, Beijing 100049, China;
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received Date: 11 May 2018
Revised Date: 15 August 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 81530094).

The protective effect of Ding-Zhi-Xiao-Wan (DZXW) on Aβ_25-35 induced PC12 cells was investigated from two aspects of cell apoptosis and oxidative stress. And the formula was decomposed into single herbs and three herbs to clarify the mechanisms of action, compatibility mechanisms and main active ingredients of DZXW. The viability of PC12 cells was evaluated by MTT assay and lactate dehydrogenease content, ginseng and poria cocos played the key roles in enhancing cell viability. Compared with the model group, it could improve the cell viability about 30%. The protection mechanism of DZXW against Aβ_25-35 induced PC12 cells injury on oxidative stress and cell apoptosis pathways were studied by flow cytometry, mitochondrial membrane potential (MMP), content of malondialdehyde (MDA) and glutathione (GSH) for the further research. Ginseng and poria could significantly inhibit the cell apoptosis, and also decreased MDA content to reduce the membrane lipid peroxidation. The effect of polygala radix (PR) and acorus tatarinowii rhizoma (ATR) could improve the content of GSH in PC12 cells to inhibit oxidative stress injuries, and the activities of ginseng and poria were improved after the compatibility with PR and ATR. Overall speaking, the compatibility of DZXW formula was scientific rationality.
- Ding-Zhi-Xiao-Wan formula,
- Decompose prescription,
- Compatibility interaction,
- Aβ_25-35,
- PC12 cell,
- Cell apoptosis,
- Oxidative stress
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
41. [41]
1. [1]
  Xinyu ZENG , Guhua TANG , Jianming OUYANG . Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth, aggregation and cell adhesion of calcium oxalate crystals. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1563-1576. doi: 10.11862/CJIC.20230374
2. [2]
  Zhaoxin LI , Ruibo WEI , Min ZHANG , Zefeng WANG , Jing ZHENG , Jianbo LIU . Advancements in the construction of inorganic protocells and their cell mimic and bio-catalytical applications. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2286-2302. doi: 10.11862/CJIC.20240235
3. [3]
  Shuyu Liu , Xiaomin Sun , Bohan Song , Gaofeng Zeng , Bingbing Du , Chongshen Guo , Cong Wang , Lei Wang . Design and Fabrication of Phospholipid-Vesicle-based Artificial Cells towards Biomedical Applications. University Chemistry, 2024, 39(11): 182-188. doi: 10.12461/PKU.DXHX202404113
4. [4]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
5. [5]
  Shipeng WANG , Shangyu XIE , Luxian LIANG , Xuehong WANG , Jie WEI , Deqiang WANG . Piezoelectric effect of Mn, Bi co-doped sodium niobate for promoting cell proliferation and bacteriostasis. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1919-1931. doi: 10.11862/CJIC.20240094
6. [6]
  Peng GENG , Guangcan XIANG , Wen ZHANG , Haichuang LAN , Shuzhang XIAO . Hollow copper sulfide loaded protoporphyrin for photothermal-sonodynamic therapy of cancer cells. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1903-1910. doi: 10.11862/CJIC.20240155
7. [7]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
8. [8]
  Hailian Tang , Siyuan Chen , Qiaoyun Liu , Guoyi Bai , Botao Qiao , Fei Liu . Stabilized Rh/hydroxyapatite Catalyst for Furfuryl Alcohol Hydrogenation: Application of Oxidative Strong Metal-Support Interactions in Reducing Conditions. Acta Physico-Chimica Sinica, 2025, 41(4): 100036-. doi: 10.3866/PKU.WHXB202408004
9. [9]
  Zhiwen HU , Weixia DONG , Qifu BAO , Ping LI . Low-temperature synthesis of tetragonal BaTiO₃ for piezocatalysis. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 857-866. doi: 10.11862/CJIC.20230462
10. [10]
  Yanhui XUE , Shaofei CHAO , Man XU , Qiong WU , Fufa WU , Sufyan Javed Muhammad . Construction of high energy density hexagonal hole MXene aqueous supercapacitor by vacancy defect control strategy. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1640-1652. doi: 10.11862/CJIC.20240183
11. [11]
  Hao Zhao , Zhen Gao , Weihong Li . Practice and Exploration of the Construction of Experimental Technician Teams of Universities in the New Period. University Chemistry, 2024, 39(4): 7-12. doi: 10.3866/PKU.DXHX202310122
12. [12]
  Runze Xu , Rui Liu . U-Pb Dating in the Age of Dinosaurs. University Chemistry, 2024, 39(9): 243-247. doi: 10.12461/PKU.DXHX202404083
13. [13]
  Qizhi Yao , Gu Jin , Pingping Zhu . Modular Analytical Chemistry Experimental Teaching Based on “Comprehensive + Exploratory” Experiments: “One Student, One Plan”, Individualized Experimental Teaching Method. University Chemistry, 2024, 39(3): 143-148. doi: 10.3866/PKU.DXHX202309071
14. [14]
  Linhan Tian , Changsheng Lu . Discussion on Sextuple Bonding in Diatomic Motifs of Chromium Family Elements. University Chemistry, 2024, 39(8): 395-402. doi: 10.3866/PKU.DXHX202401056
15. [15]
  . Cover and Table of Contents for Vol.40 No. 12. Acta Physico-Chimica Sinica, 2024, 40(12): -.
16. [16]
  Haiming Wu , Gaya N. Andrew , Rajini Anumula , Zhixun Luo . Corrigendum to 'How ligand coordination and superatomic-states accommodate the structure and property of a metal cluster: Cu₄ (dppy)₄ Cl₂ vs. Cu₂₁ (dppy)₁₀ with altered photoluminescence' [Chin. Chem. Lett. 35 (2024) 108340]. Chinese Chemical Letters, 2024, 35(12): 109912-. doi: 10.1016/j.cclet.2024.109912
17. [17]
  Changqing MIAO , Fengjiao CHEN , Wenyu LI , Shujie WEI , Yuqing YAO , Keyi WANG , Ni WANG , Xiaoyan XIN , Ming FANG . Crystal structures, DNA action, and antibacterial activities of three tetranuclear lanthanide-based complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2455-2465. doi: 10.11862/CJIC.20240192
18. [18]
  Wenyan Dan , Weijie Li , Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060
19. [19]
  Yifeng Xu , Jiquan Liu , Bin Cui , Yan Li , Gang Xie , Ying Yang . “Xiao Li’s School Adventures: The Working Principles and Safety Risks of Lithium-ion Batteries”. University Chemistry, 2024, 39(9): 259-265. doi: 10.12461/PKU.DXHX202404009
20. [20]
  Zhifang SU , Zongjie GUAN , Yu FANG . Process of electrocatalytic synthesis of small molecule substances by porous framework materials. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2373-2395. doi: 10.11862/CJIC.20240290

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(840)
HTML views(86)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on Compatibility Mechanism of Ding-Zhi-Xiao-Wan Formula in Treating Alzheimer's Disease Based on Aβ25-35 Induced PC12 Cells

Metrics

通讯作者: 陈斌, bchen63@163.com

Study on Compatibility Mechanism of Ding-Zhi-Xiao-Wan Formula in Treating Alzheimer's Disease Based on Aβ_25-35 Induced PC12 Cells