Citation: CHENG Dan, GUO Yan-Li, KANG Xiao-Feng. Determination of Antituberculosis Drug Isoniazid by Nanopore-based Sensor[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(8): 1186-1192. doi: 10.11895/j.issn.0253-3820.181296 shu

Determination of Antituberculosis Drug Isoniazid by Nanopore-based Sensor

Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China

Received Date: 5 May 2018
Revised Date: 8 June 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21327806).

An analytical method was proposed for determination of antituberculosis drug isoniazid at single-molecule level by protein nanopores equipped with a cyclodextrin adapter. The developed method is rapid, highly sensitive and specific. The influence of channel protein, pH of buffer solution and recording voltage on detection signal was investigated. The results showed that (M113R)₇ could provide an enhanced resolution for isoniazid recognition compared with that observed in wild-type protein or (M113N)₇. The addition of isoniazid from cis side during relatively high voltage and neutral pH conditions could improve the detection sensitivity. Some common pharmaceutical excipients (glucose, sucrose and starch), metal ions that may coexist in solution and synthetic intermediates of isoniazid, isonicotinic acid (the concentrations of all analytes were above 40 times of isoniazid) were added to evaluate the selectivity of the sensing platform, and it was found that no obvious response could be observed after adding these analytes mentioned above, showing a good selectivity. The linear range and the detection limit of the sensor were 0.5-30 μmol/L and 2 nmol/L. The method was directly applied to the rapid detection of isoniazid, with the recoveries of 92.9%-108.2%.
- α-Hemolysin,
- Nanopore detection,
- Cyclodextrin,
- Isoniazid
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