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Citation: ZHANG Hong-Yan, ZHOU Ye, WU Ren-An. In-situ Proteomic Characterization of Protein Conformation on Nanoparticle Surface by Cross-linking Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(10): 1570-1580. doi: 10.11895/j.issn.0253-3820.181290 shu

In-situ Proteomic Characterization of Protein Conformation on Nanoparticle Surface by Cross-linking Mass Spectrometry

ZHANG Hong-Yan ^1,2, ,
ZHOU Ye ^1,2 ,
WU Ren-An ¹

1.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 3 May 2018
Revised Date: 19 July 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21375125, 21675156).

The conformation change of bovine serum albumin (BSA) absorbed on Fe₃O₄ nanoparticle surface was characterized by chemical cross-linking mass spectrometry (CXMS)-based proteomics approach. The effects of nanoparticle surface curvatures and initial protein concentrations on protein conformation change were investigated by the CXMS. The results revealed that the BSA adsorbed on the surface of Fe₃O₄ nanoparticle could undergo an altered conformational state in comparison to that of BSA in solution as the crosslinking frequency of some amino acid residues of the BSA varied significantly, with the assistant analysis of the attenuated total reflectance Fourier transfer infrared spectroscopy (ATR-FTIR). By virtue of the high-throughput, high accuracy and high sensitivity of mass spectrometry, the CXMS-based proteomic method could provide structural information of proteins adsorbed on nanoparticle surface by crosslinking restraints and would be promised to be applied to the analysis of nano-bio interaction for proteins in complex biological systems.
- Chemical cross-linking mass spectrometry,
- Proteomics,
- Nanoparticle,
- Protein conformation
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
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