Citation: ZHANG Lian-Ming, ZHANG Dong-You, ZENG Ying, LI Jian-Ping. A Cimaterol Molecularly Imprinted Sensor Based on DNA-assisted Recognition[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(11): 1770-1777. doi: 10.11895/j.issn.0253-3820.181269 shu

A Cimaterol Molecularly Imprinted Sensor Based on DNA-assisted Recognition

¹ Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China;
² College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China

Received Date: 24 April 2018
Revised Date: 28 June 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21765006), the Natural Science Foundation of Guangxi Province of China (No.2015GXNSFFA139005), the Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, and Foundation of Guilin University of Technolgy (No. GUTQDJJ2016024).

A novel strategy based on DNA induced conformation change was developed by rendering the imprinting cavity with refined structure in order to further improve the selectivity of molecular imprinting technology. The double-stranded DNA (dsDNA) was first self-assembled on the surface of the gold electrode, then the cimaterol molecule, taken as the representative target, was bound with the groove of dsDNA to form the complex template, together with which the molecular imprinting polymer (MIPs) was prepared by electropolymerization in the monomer solution. After elution of cimaterol, the obtained MIPs sensor had a remarkably high selectivity toward cimaterol. The combination process of cimetaerol and DNA was studied by UV-Vis and it was found to be the groove combination model. The optimum conditions of the system were optimized by electrochemical method. The results showed that when using ethanol-acetic acid (8:1, V/V) as an eluent, with the elution time of 35 min and rebound time of 45 min, the sensor showed the best recognition ability toward cimaterol. This sensor exhibited a linear detection range of 1.0×10^-13-1.0×10^-10 mol/L, with the detection limit of 3.2×10^-14 mol/L. The sensor was successfully applied to cimaterol detection in pork samples.
- Molecular imprinting,
- Cimaterol,
- Electrochemical sensor,
- DNA,
- Selective recognition
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