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Citation: JIN Can, YANG Xi, XU Shu-Juan, JI Yi-Bing. Preparation of Bovine Serum Albumin-modified Organic-silica Hybrid Monolithic Columns and Its Electrochromatography Study[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(10): 1637-1643. doi: 10.11895/j.issn.0253-3820.181166 shu

Preparation of Bovine Serum Albumin-modified Organic-silica Hybrid Monolithic Columns and Its Electrochromatography Study

College of Science, China Pharmaceutical University, Nanjing 210009, China

Received Date: 16 March 2018
Revised Date: 15 June 2018

An epoxy functionalized organic-silica hybrid monolith was successfully synthesized via one-step method with tetramethoxysilane (TMOS) and γ-glycidyloxypropyltrimethoxysilane (GPTMS) as precursors. The volume of reactive monomer was optimized and the capillary monolithic columns with good permeability and mechanical strength were obtained. Scanning electron microscope characterization demonstrated that a structure of continuous bed was formed. Then bovine serum albumin (BSA) was covalently immobilized on capillary monolith inner surface by Schiff base method. The preparation condition was studied and it was found that the appropriate concentration of BSA for preparation of BSA-modified hybrid monoliths was 8.0 g/L. Meanwhile, the influence factors on the sample separation were investigated, including running buffer pH (25 mmol/L Tris, pH 7.4), applied voltage (10 kV), capillary temperature (15℃) and sample condition (2 kV×3 s). The BSA-modified hybrid monolith exhibited good chiral separation ability to (±)-tryptophan and achieved satisfactory run-to-run, day-to-day and column-to-column repeatability. Compared with BSA-immobilized silica-based monolithic column, this work presented better chiral recognition ability and also simplified preparation procedures and shortened preparation period.
- Organic-silica hybrid monolith,
- Bovine serum albumin,
- Capillary electrochromatography,
- Chiral stationary phase
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