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Citation: HAN Yu-Ping, SHEN Lin, LI Zhen, HU Cheng-Guo, LIU Zhi-Hong. An Aptasensor Based on Upconversion Nanoparticles-Polydopamine Nanoparticles Nanosystem for Detection of Carcinoembryonic Antigen[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(8): 1178-1185. doi: 10.11895/j.issn.0253-3820.181119 shu

An Aptasensor Based on Upconversion Nanoparticles-Polydopamine Nanoparticles Nanosystem for Detection of Carcinoembryonic Antigen

Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China

Received Date: 15 February 2018
Revised Date: 22 May 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21575109).

An aptasensor was constructed based on upconversion fluorescence resonance energy transfer (UC-FRET) between sandwich-structure upconversion nanoparticles (SWUCNPs, donor) and polydopamine nanoparticles (PDA NPs, receptor). With the π-π stacking between PDA NPs and carcinoembryonic antigen (CEA) aptamer which was covalently coupled to polyacrytic acid modified SWUCNPs, the fluorescence of SWUCNPs could be quenched up to 95%. After introducing CEA, the aptamer preferentially combined with CEA, which blocked the energy transfer process between SWUCNPs and PDA NPs, and thus the fluorescence of SWUCNPs was recovered. Under the optimized experimental conditions, the fluorescence restoration of SWUCNPs was linearly related to CEA concentration ranging from 1.0 ng/mL to 250.0 ng/mL in both buffer solution and human serum samples. The aptasensor exhibited good selectivity for CEA bearing little interference from other substances. Simple synthesis, good quenching ability, water solubility and label-free property ensure PDA NPs as a kind of alternative energy acceptor of UCNPs, which will extend the applications of UC-FRET biosensing platform.
- Upconversion nanoparticles,
- Polydopamine nanoparticles,
- Carcinoembryonic antigen,
- Aptamer
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