Citation: LI Ran, LIN Ze-Jia, YANG Jin-Yi, XU Zhen-Lin, WANG Hong, LEI Hong-Tao, SUN Yuan-Ming, SHEN Yu-Dong. An Indirect Competitive Enzyme-linked Immunosorbent Assay for Simultaneous Determination of Florfenicol and Thiamphenicol in Animal Meat and Urine[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(8): 1321-1328. doi: 10.11895/j.issn.0253-3820.181084 shu

An Indirect Competitive Enzyme-linked Immunosorbent Assay for Simultaneous Determination of Florfenicol and Thiamphenicol in Animal Meat and Urine

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China

Received Date: 31 January 2018
Revised Date: 18 May 2018

Fund Project: This work was supported by the Science and Technology Planning Project of Guangzhou (No. 201704020082), the National Key Research and Development Program of China (No. 2016YFE0106000), Science and Technology Planning Project of the Guangxi Zhuang Autonomous Region (No.2017AB47020), the National Natural Science Foundation of China (No. 31371769) and Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2017).

A high-affinity polyclonal antibody was prepared by immunizing animals with haptens FFD and FFM. Under the optimal combination of coating antigen and antibody, an indirect competitive enzyme-linked immunosorbent assay (icELISA) for simultaneously detecting florfenicol and thiamphenicol residues in animal meat and urine samples was developed. The icELISA showed an IC₅₀ value of 1.32 ng/mL for florfenicol and 2.13 ng/mL for thiamphenicol, respectively. The linear ranges were from 0.31 to 5.61 ng/mL with a limit of detection of 0.12 ng/mL for florfenicol, and 0.41 to 11.2 ng/mL with a limit of detection of 0.15 ng/mL for thiamphenicol, respectively. The average recoveries of florfenicol and thiamphenicol in spiked samples ranged from 77.2% to 116.0% with a relative standard deviation of less than 15%. Therefore, this proposed icELISA provided a valid detection for florfenicol and thiamphenicol residues in animal meat product and urine samples.
- Florfenicol,
- Thiamphenicol,
- Antibody,
- Indirect competitive enzyme-linked immunosorbent assay
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
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