Citation: CHEN Lu-Lu, WANG Zhong-Hua, ZHOU Zhi, HE Bing-Shu, HE Jiu-Ming, HUANG Luo-Jiao, NURBOLAT Aidarhan, LIU Ge-Yu, HAJI Akber Aisa, ABLIZ Zeper. Development of Plant Metabolomics Analytical Approach Based on Liquid Chromatography Tandem Mass Spectrometry in Artemisia rupestris L.[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(5): 735-742. doi: 10.11895/j.issn.0253-3820.171489 shu

Development of Plant Metabolomics Analytical Approach Based on Liquid Chromatography Tandem Mass Spectrometry in Artemisia rupestris L.

¹ Centre for Imaging & Systems Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China;
² State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;
³ Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China

Received Date: 7 December 2017
Revised Date: 27 February 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 2167050718).

Due to the diversity and complexity, the change of chemical components in medicinal plant according to the time, cultivated varieties or ecological condition is difficult to recognize using traditional phytochemistry method. In order to analyze the pharmacodynamics material basis in Uighur medicinal plant Artemisia rupestris L. in an effective and comprehensive way, a plant metabolomics approach was established based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). This study firstly focused on the effect of extraction solvents, redissolve solvents and ultrasonic time on the untargeted metabolomics, then the optimal preparation condition was selected according to metabolites coverage. After methodology validation, the approach was applied to acquire metabolic information in root, stem, branchlet, leaf and flower of Artemisia rupestris L. The results showed that the metabolome in flower was obviously different with the other organs. Coupling with multivariate statistical analysis, a batch of differential metabolites were picked out, in which 61 flavonoids, 97 rupestonic acid derivatives, 7 chlorogenic acids and 15 other compounds were primarily recognized according to the characteristic fragmentation rules of specific structure type and database retrieval. Additionally, the distribution characteristics of the above 180 differential metabolites was illustrated by cluster heat map. In conclusion, this study provided important information about the rational utilization of effective parts from Artemisia rupestris L., and offered a novel strategy for quality control, variety improvement and reasonable development of medicinal plants.
- Artemisia rupestris L.,
- Liquid chromatography-tandem mass spectrometry,
- Plant metabolomics,
- Sample preparation,
- Multivariate statistical analysis
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