Citation: SUN Yan, XIAO Nan, LI Guang, HAN Yan-Yan, LIU Shu-Ying, WANG En-Peng, CHEN Chang-Bao. A Comparative Pharmacokinetic Study of Ginsenoside Re after Oral Administration in Normal and Ultraviolet B Irradiation-induced Damage Rats[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(5): 678-683. doi: 10.11895/j.issn.0253-3820.171136 shu

A Comparative Pharmacokinetic Study of Ginsenoside Re after Oral Administration in Normal and Ultraviolet B Irradiation-induced Damage Rats

Changchun University of Chinese Medicine, Jilin Ginseng Academy, Changchun 130117, China

Received Date: 28 July 2017
Revised Date: 2 March 2018

Fund Project: This work was supported by the Jilin Provincial Science and Technology Department, China and Changchun University of Chinese Medicine (Nos. 20170520041JH, 20160307021YY).

A methodology of quantitative analysis on ginsenoside Re (G-Re) in rat plasma by ultra performance liquid chromatography-triple quadrupole mass spectrometry was developed for comparing the pharmacokinetic profiles between normal rats and Ultraviolet B (UVB) irradiation-induced damage rats after oral administration. The sample separation was carried out on an Ascentis® Express C₁₈ column (5.0 mm×3.0 mm, 2.7 μm) with 0.1% formic acid in water and acetonitrile as the mobile phase under gradient elution. MS analysis was operated in multiple-reaction monitoring (MRM) mode using electrospray ionization (ESI) with negative ion mode, and the ions for quantification were m/z 991.54/945.53/475.60. The limit of detection (LOD, S/N=3), limit of quantification (LOQ, S/N=10) were 4.0 ng/mL and 13.5 ng/mL, respectively. G-Re was in good linearity between 15 ng/mL and 20000 ng/mL (r=0.999), the intra-day and inter-day precisions, recovery, matrix effect and stability could meet the pharmacokinetic analysis requirement. The results indicated that the metabolic process of G-Re conformed to a two-compartment pharmacokinetic model after single oral administration in the normal and model groups. The t_1/2α were (0.21±0.04) h and (0.69±0.07) h, respectively; t_1/2β were (17.08±0.53) and (21.40 ±16.77) h, respectively; AUC_(0-t) were (321.91±2.27) μg/(L·h) and (474.99±194.96) μg/(L·h), respectively; AUC_(0-∞) were (332.44±1.66) μg/(L·h) and (518.64±231.39) μg/(L·h), respectively; the pharmacokinetic parameters were significantly different between normal and UVB irradiated rats (p<0.05), except for t_1/2α. This UHPLC-QQQ-MS method showed excellent separation, accuracy, high sensitivity, specificity and good repeatability, and it was suitable for the pharmacokinetic study of G-Re in vivo.
- Ginsenoside Re,
- Ultraviolet B Radiation,
- Pharmacokinetics
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