Citation: ZHAO Fang, LI Qiang, LIANG Mei-Li, YAN Yan, XING Jie, QIN Xue-Mei, GAO Xiao-Xia. Simultaneous Determination of Eight Kinds of Neurotransmitters in Rat Blood Serum by Ultra-high Performance Liquid Chromatograph- Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(1): 121-128. doi: 10.11895/j.issn.0253-3820.171115 shu

Simultaneous Determination of Eight Kinds of Neurotransmitters in Rat Blood Serum by Ultra-high Performance Liquid Chromatograph- Tandem Mass Spectrometry

ZHAO Fang ^1, ,
LI Qiang ^1,2 ,
LIANG Mei-Li ^1,2 ,
YAN Yan ¹ ,
XING Jie ¹ ,
QIN Xue-Mei ¹ ,
GAO Xiao-Xia ¹

1.
Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China;2 Chemistry & Chemical Engineering of Shanxi University, Taiyuan 030006, China

Received Date: 21 July 2017
Revised Date: 25 September 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.81473415), the Program for the Outstanding Innovative Teams of Higher Learning Institutions of Shanxi Province, Outstanding Youth Foundation Project of Applied Basic Research Project of Shanxi Province(No.201701D211009), the Focus of The Shanxi Provincial Science and Technology Innovation Project Team (No. 201605D131045-18) and the Shanxi Provincial Key Laboratory of Efficacy and Utilization of Traditional Chinese Medicine (No.201605D111004).

An ultra-high performance liquid chromatography tandem mass spectrometric (LC-MS/MS) method for simultaneous determination of 8 kinds of neurotransmitters (5-HT, GABA, Glu, ACH, NE, DA, 5-HIAA, HVA) in rat serum was developed. The blood samples were extracted by 0.1% formic acid in acetonitrile and separated on a Waters ACQUITY UPLC BEH C₁₈ (2.1 mm×100 mm, 1.7 μm) using gradient elution, with the mobile phase consisting of acetonitrile and 0.1% formic acid in water. The samples were then ionized with positive electrospray (ESI⁺), and detected under multiple reaction monitoring (MRM) mode. As a result, 8 kinds of neurotransmitters were determined accurately in 10 min with a limit of quantitation of 1.8 ng/mL and intra-day and inter-day precisions of ≤ 9.2% (n=6). This method showed a good linearity in detection of neurotransmitters and the linear correlation coefficients were greater than 0.994. Also the stability, recovery and matrix effect were eligible for the analysis. This method showed high accuracy, sensitivity, strong specificity, good stability, small matrix effect and short time for analysis, and was suitable for the quantitative determination of monoamine, amino acids and acetylcholine neurotransmittes in rat serum.
- Ultra-high performance liquid chromatograph-tandem mass spectrometry,
- Neurotransmitters,
- Serum,
- Determination
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