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Citation: KONG Jing-Lin, LIU Wei-Wei, DING Jun-Jie, ZHANG Lin, LI Bao-Qiang, QIN Mo-Lin, CHEN Chuang, LI Hai-Yang. Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(11): 1583-1588. doi: 10.11895/j.issn.0253-3820.171052 shu

Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry

1.
Institute of Chemical Defense, Beijing 102205, China;
2.
Dalian Institute of Chemical Physics Chinese Academy of Sciences, Dalian 116023, China

Received Date: 30 June 2017
Revised Date: 8 September 2017

The electrospray ionization-ion mobility spectrometric (ESI-IMS) technique has the potential as an analytical separation tool in analyzing polypeptides and amino acids for fast screening unknown samples in anti-chemical and biological terror attacks. A method for detecting several polypeptides and amino acids was developed based on ESI-IMS using air as drift gas at room temperature. The ion mobility of four amino acids and two polypeptides dissolved in methanol was determined on the system at elution rate of 2μL/min. The spectra of these compounds had characteristics of finger-printing maps. The limit of detection of this instrument for Substance P could reach 855 ng/mL in 1 min. The results showed that a small, self-contained ESI-IMS instrument with reservoirs of air could be used to quickly detect and accurately identify polypeptides and amino acids.
- Electrospray,
- Ion mobility spectrometry,
- Amino acid,
- Polypeptide,
- Reduced mobility
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1.
沈阳化工大学材料科学与工程学院沈阳 110142

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