Citation: LIN Dong-Guo, LIN Jin-Qiong, LI Pei-Wen, YANG Na, XU Bang-Lao, LIU Da-Yu. Construction of Tumor Tissue Array on An Open-Access Microfluidic Chip[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(1): 113-120. doi: 10.11895/j.issn.0253-3820.171046 shu

Construction of Tumor Tissue Array on An Open-Access Microfluidic Chip

LIN Dong-Guo ^1,2,3, ,
LIN Jin-Qiong ² ,
LI Pei-Wen ² ,
YANG Na ^2,3 ,
XU Bang-Lao ^2,3 ,
LIU Da-Yu ^1,2,3

1.
Laboratory of Clinical Chemistry, The Second Hospital of South China University of Technology, Guangzhou 510180, China;2 Department of Laboratory Medicine, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China;3 Clinical Molecular Medicine and Molecular Diagnosis Key Laboratory of Guangdong Province, Guangzhou 510180, China

Received Date: 29 June 2017
Revised Date: 18 October 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 81371649, 81171418, 81602434) and the Guangzhou Science Technology and Innovation Comission (Nos. 20141A010007,[2016]171).

An open-access microfluidic chip which enabled automatic cell distribution and complex multi-step operations was developed. The microfluidic chip featured a key structure in which a nanoporous membrane was sandwiched by a cell culture chamber array layer and a corresponding media reservoir array layer. The microfluidic approach took advantage of the characteristics of the nanoporous membrane. On one side, this membrane permitted the flow of air but not liquid, thus acting as a flow-stop valve to enable automatic cell distribution. On the other side, it allowed diffusion-based media exchange and thus, mimicked the endothelial layer. In synergy with a liquid transferring platform, the open-access microfluidic system enabled complex multi-step operations involving medium exchange, drug treatment, and cell viability testing. By using this microfluidic protocol, a 10×10 tissue arrays was constructed in 90 s, followed by schedule-dependent drug testing. Morphological and immunohistochemical assays results indicated that the resultant tumor tissue was faithful to that in vivo. Drug testing assays showed that the microfluidic tissue array promised multi-step cell assays under biomimetic microenvironment, thus providing an advantageous tool for cell research.
- Open-access microfluidic chip,
- Nanoporous membrane,
- Microenvironment,
- Tissue microarray,
- Anti-cancer drug testing
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