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Citation: XIAO Hui, HE Jing-Lin, XIAO Hao, YANG Chan, FENG Ze-Meng, YIN Yu-Long, CAO Zhong. Terminal Deoxynucleotidyl Transferase Amplification Based DNA-Copper Nanoclusters Sensor for Detection of L-Histidine[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(10): 1517-1522. doi: 10.11895/j.issn.0253-3820.170336 shu

Terminal Deoxynucleotidyl Transferase Amplification Based DNA-Copper Nanoclusters Sensor for Detection of L-Histidine

XIAO Hui ^1, ,
HE Jing-Lin ¹ ,
XIAO Hao ² ,
YANG Chan ¹ ,
FENG Ze-Meng ² ,
YIN Yu-Long ² ,
CAO Zhong ¹

1.
Collaborative Innovation Center of Micro/Nano Bio-sensing and Food Safety Inspection, Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410114, China;2 Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received Date: 26 May 2017
Revised Date: 22 August 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.31527803, 21545010, 21645009).

A terminal deoxynucleotidyl transferase (TdT) amplification based DNA-copper nanoclusters (CuNCs) sensor was developed for detection of L-histidine (L-His). Single strand DNA containing poly-thymine (T) sequences were synthesized by TdT in the presence of dTTP. In blank control, poly-T sequences worked as templates of CuNCs due to the affinity between thymine and copper ions(Ⅱ). Fluorescence intensity was enhanced when CuNCs formed with reducing agents. In the presence of L-His, the imidazolyl group of L-His worked as a chelating agent that formed L-His-Cu²⁺ chelated complex. Thus less copper ions were induced in poly-T sequences, and less CuNCs were obtained to produce week fluorescence signals. A good linear correlation was obtained between fluorescence change and the logarithm of the L-His concentration over the range of 5.0×10^-9-5.0×10^-4 mol/L. The detection limit was estimated as 3.4×10^-9 mol/L. And the recoveries were 97.4%-104.6% for the actual urine samples. Compared with other methods of synthetic CuNCs, this method allowed to specifically determining L-histidine without template or labeling, which showed good potential in biomedical and clinical analysis.
- Terminal deoxynucleotidyl transferase,
- Poly-thymine,
- Copper nanoclusters,
- L-Histidine,
- Fluorescence analysis
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