Citation: SUN Zhi-Hong, ZHOU Li-Hua, DENG Guan-Jun, ZHEGN Ming-Bin, YAN Wei-Qiang, LI Wen-Jun, CAI Lin-Tao, GONG Ping. Tumor Targeting of Fluorescent Magnetic IR780-Fe₃O₄ Nanoparticles for Detection of Circulating Tumor Cells[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(10): 1427-1433. doi: 10.11895/j.issn.0253-3820.170280 shu

Tumor Targeting of Fluorescent Magnetic IR780-Fe₃O₄ Nanoparticles for Detection of Circulating Tumor Cells

SUN Zhi-Hong ^1, ,
ZHOU Li-Hua ¹ ,
DENG Guan-Jun ¹ ,
ZHEGN Ming-Bin ^1,2,3 ,
YAN Wei-Qiang ⁴ ,
LI Wen-Jun ¹ ,
CAI Lin-Tao ¹ ,
GONG Ping ^1,2,3

1.
Nano Special Pharmaceutical Engineering Laboratory, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China;2 Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Dongguan 523808, China;3 State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Normal University, Changsha 410081, China;4 Department of Radiology, Peking University Shenzhen Hospital, Shenzhen 518036, China

Received Date: 4 May 2017
Revised Date: 22 June 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21375141, 81671758) and Shenzhen Science and Technology Program (Nos. JSGG20160331185422390, JCYJ20150403091443298, KQCX20140521115045447, JCYJ20160429191503002).

The tumor targeted fluorescent magnetic IR780-Fe₃O₄ nanoparticles were prepared for separation and detection of circulating tumor cells (CTCs). These IR780-Fe₃O₄ nanoparticles were characterized by electron microscopy, fluorescence spectrometer, and superconducting quantum interferometer. The targeting effect of IR780-Fe₃O₄ nanoparticles was analyzed on the tumor and normal cells by confocal microscope and flow cytometry, and the confocal microscope was used to target the location of IR780-Fe₃O₄ nanoparticles in MCF-7 cells. The standard curve was drawn and evaluated accorded to the IR780-Fe₃O₄ nanoparticles fluorescence intensity of tumor cells after incubation. The results showed that IR780-Fe₃O₄ nanoparticles could target a variety of CTCs. Furthermore, cellular localization experiment proved that IR780-Fe₃O₄ nanoparticles could target the mitochondria of tumor cells. With the method of coupling magnetic Fe₃O₄ nanoparticles, IR780 could well distinguish the tumor and normal cells, which could be used for separating and detecting the CTCs in simulated blood.
- Circulating tumor cells,
- Fluorescence intensity,
- IR780,
- Fe₃O₄ magnetic nanoparticles
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Tumor Targeting of Fluorescent Magnetic IR780-Fe₃O₄ Nanoparticles for Detection of Circulating Tumor Cells