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Citation: GE Wen-Qi, SHENG Ying-Ying, QIAO Ya-Dong, CAO Zhong, WU Hai-Chen, YAO Zhi-Yi. Fluorescent Detection of p-Nitroaniline Based on an Anionic Pyrenyl Probe[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(10): 1523-1528. doi: 10.11895/j.issn.0253-3820.170263 shu

Fluorescent Detection of p-Nitroaniline Based on an Anionic Pyrenyl Probe

1.
Collaborative Innovation Center of Micro/Nano Bio-sensing and Food Safety Inspection, Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410114, China;2 Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;3 College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Received Date: 26 April 2017
Revised Date: 19 June 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21275022, 21545010)

p-Nitroaniline (PNA) was one kind of highly toxic aromatic amine and becomes an environmental pollutant in recent years. Here we reported the construction of a fluorescent sensor based on an anionic pyrene, 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS), for the rapid and visual detection of PNA in aqueous media. The fluorescence quenching of HPTS caused by the presence of PNA was through non-covalent interactions. A good linear relationship between fluorescent intensity of HPTS at 512 nm was obtained in the range of 10-120 μmol/L. The detection limit (3σ) of this approach was 4.6 μmol/L. The results showed that the method was suitable for rapid detection of PNA in real samples with good sensitivity, selectivity, anti-interference, low cost and easy operation.
- Pyrene,
- Fluorescent probe,
- Sensor,
- p-Nitroaniline
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