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Citation: XIE Jing-Meng, MENG Chen-Ying, LI Er-Fen, ZHANG Ying-Xia, SONG Xu-Qin, ZHANG Mei-Yu, HE Li-Min. Simultaneous Determination of Ten Banned Substances in Pig Urine by Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(5): 750-756. doi: 10.11895/j.issn.0253-3820.170127 shu

Simultaneous Determination of Ten Banned Substances in Pig Urine by Liquid Chromatography-Tandem Mass Spectrometry

¹ National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
² Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China

Received Date: 3 May 2017
Revised Date: 25 February 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31372476).

A simple, sensitive and reliable method was developed for simultaneous determination of ten banned drugs residues including zeranols (ZALs), chloroamphenicol, pentachlorophenol, etc. in swine urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The urine samples were pretreated using lyophilization and QuEChERS procedures, respectively. Acetonitrile and ammonium acetate (5 mmol/L) were chosen as mobile phases. Target compounds were separated well in ZorbaxSB-C₁₈ by following the optimized gradient elution program and determined by LC-MS/MS in negative electrospray ionization mode. The linearity of the matrix-matched standard curve of ten analytes in two methods was good in the range of the experimental concentration with correlation coefficients more than 0.99. The recoveries of ten drugs were in the range of 80.7%-107.7% and 73.5%-103.3% at the spiked levels of 5, 10 and 20 μg/L by lyophilization and QuEChERS methods, respectively. The coefficients of variation were less than 15%. The limits of detection (LOD) and the limits of quantification (LOQ) from lyophilization and QuEChERS method were 0.1 to 2.0 μg/L and 0.2 to 5.0 μg/L, respectively.
- Banned substances,
- Lyophilization method,
- Liquid chromatography-tandem mass spectrometry,
- Swine urine,
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