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Citation: XU Feng-Jie, BIAO Lin-Hai, ZU Yuan-Gang, LIU Zhi-Guo. Study on Interactions between Paeonol and Cadherin[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(7): 1025-1030. doi: 10.11895/j.issn.0253-3820.160877 shu

Study on Interactions between Paeonol and Cadherin

Key Laboratory of Forest Plant Ecology of Ministry of Education, State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China

Received Date: 1 December 2016
Revised Date: 22 March 2017

Paeonol has inhibitory effect on a variety of tumor cells, whereas cadherin is a kind of glycoprotein that is associated with the occurrence and development of different tumor. In this study, the interactions of paeonol and E-cadherin have been investigated by fluorescence spectrometry and atomic force microscopy (AFM). Fluorescence spectrometry results revealed that the addition of paeonol significantly quenched the fluorescence of E-cadherin. Based on the results of the quenching constant, it was inferred that the interaction of paeonol and E-cadherin was a static quenching process. The thermodynamic parameters ΔH and ΔS were calculated to be -4.3×10⁵ J/mol and -1.3×10³ J/(mol·K), respectively, which proved the involvement of weak interactive forces such as hydrogen bond van der Waals force. AFM results revealed that cadherin molecules were assembled into the long-chain structure. The addition of paeonol could significantly disrupt these assembling structures into short chains, which could be ascribed to the damage of the interdigitation model from the adjacent cadherin molecules. All these results reveal that cadherin is an important target of paeonol to modulate its activity.
- Paeonol,
- E-cadherin,
- Fluorescence spectrometry,
- Atomic force microscopy
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