Citation: WANG Zhong-Hua, CHEN Yan-Hua, XU Jing, CHEN Hui-Li, ZHOU Xia, XIAO Xin-Hua, PING Fan, HE Jiu-Ming, ABLIZ Zeper, ZHANG Rui-Ping. Comparison of Multiple Ionization Methods Coupled with Liquid Chromatography-Mass Spectrometry in Lipidomics Study of Serum Samples[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(5): 674-680. doi: 10.11895/j.issn.0253-3820.160863 shu

Comparison of Multiple Ionization Methods Coupled with Liquid Chromatography-Mass Spectrometry in Lipidomics Study of Serum Samples

1.
State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;
2.
Centre for Imaging & Systems Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China;
3.
Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China

Received Date: 25 November 2016
Revised Date: 17 February 2017

Fund Project: This work was supported by the National High Technology Research and Development Program of China (No. 2014AA021101).

Three commonly used ionization techniques, including electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI) were compared systemically for their ionization ability, sensitivity and coverage for detecting lipids, aiming to explore their applicability in lipidomics study of serum samples. Serum samples were pretreated by liquid-liquid extraction with methyl tertiary butyl ether and then introduced into a LC-MS system equipped with electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI) source. Chromatographic separation was performed on an Ascentiss Express C8 column (150 mm×2.1 mm, 2.7 μm). A binary gradient system of acetonitrile-water (3∶2, V/V) and isoropyl alcohol-acetonitrile (9∶1, V/V), both containing 10 mmol/L ammonium formate and 0.1% formic acid, was used as eluent A and B, respectively. Data were acquired in both positive and negative ion modes for each ionization technique. The results showed that fatty acids (FFAs), phospholipids (PLs), sphingophosphalipids (SPs), glycerolipids (GLs), could be ionized most efficiently under ESI source, while APPI source gave the best sensitivity for cholesterol (CHOL), cholesterol esters (CEs) and Sphinganine (Sphingolipids, SPs). Prenol lipids could be ionized equally well under ESI and APPI source, and that the application of ESI and APPI integrated approach was expected to enhance the overall sensitivity of the method and provided more comprehensive lipids information for the global serum lipidomics study.
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