Citation: HUANG Hai-Ping, XU Liang, YUE Ya-Feng, LYU Lian-Lian. A Novel Hydrogen Peroxide Biosensor Based on Hemoglobin/Holmium Phosphate Nanocomposites[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(1): 111-117. doi: 10.11895/j.issn.0253-3820.160678 shu

A Novel Hydrogen Peroxide Biosensor Based on Hemoglobin/Holmium Phosphate Nanocomposites

School of Metallurgy and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, China

Received Date: 10 September 2016
Revised Date: 31 October 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.21465013,21005034)

The nanomaterial of n-HoPO₄ was synthesized via hydrothermal method with Ho₂O₃ as raw material, and its morphology and component were characterized by SEM and EDS. The composite material of n-HoPO₄ and hemoglobin (Hb) was dropped onto the surface of the glassy carbon electrode (GCE) so as to construct the biosensor for detection of hydrogen peroxide. The whole process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results indicated that (Hb/n-HoPO₄/GCE) possessed good electrochemical catalytic effect towards H₂O₂ reduction. The n-HoPO₄ showed good electrical conductivity and could promote the direct electron transfer rate between Hb and GCE. The effect of different pH value and electrochemical scan rates on the response current of the modified electrode was studied. Under the optimal conditions, the biosensor exhibited a good linear detection range from 50 to 1000 μmol/L (correlation coefficient R=0.999) with the detection limit of 17 μmol/L (S/N=3). The biosensor showed wide detection range, good stability and reproducibility and strong anti-interference ability, and could be used for real sample detection.
- Hemoglobin,
- Holmium phosphate,
- Hydrogen peroxide,
- Electrocatalysis,
- Biosensor
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