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Citation: GUO Ying-Ying, WU Shang-Quan, JIN Li, WANG Wan-Jun, ZHANG Qing-Chuan. Label-Free Detection of Immunoglobulin G Based On Silicon-on-Insulator Microring Resonator Analysis System Integrated with Microfluidic Channels[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(4): 483-488. doi: 10.11895/j.issn.0253-3820.160650 shu

Label-Free Detection of Immunoglobulin G Based On Silicon-on-Insulator Microring Resonator Analysis System Integrated with Microfluidic Channels

1.
Chinese Academy of Sciences, Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of China, Hefei 230027, China;
2.
China Electronics Technology Group Corporation No.38 Research Institute, Hefei 230001, China

Received Date: 2 September 2016
Revised Date: 15 February 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 1162780010, 11502265, 11472266)

The microfluidic channels integrated with microring resonator were designed. The salt coalescence on chip surface caused by liquid volatilization in open environment was avoided and only 30 μL of reaction solution was consumed. These channels significantly reduced the experiment cost. The design, fabrication and characterization of a highly sensitive and label-free silicon-on-insulator (SOI) microring optical resonator integrated with the microfluidic channels were demonstrated. The radius of the microring was 5 μm and the straight waveguide with a width of 450 nm was employed in the microring resonator. The microring resonator device had many advantages such as high sensitivity, label-free and real-time detection. Using different concentrations of ethanol solution with known refractive indices, the refractive index detection sensitivity was 76.09 nm/RIU and the volume refractive index detection limit was 5.25×10^-4 RIU. We also demonstrated the label-free quantitative specific detections of human immunoglobulin G (IgG) solutions using an antibody-modified microring resonator by measuring the resonance wavelength shift resulting from refraction index changes causing by the immobilization of antibodies and specific recognition between antibodies and antigens, respectively. The results showed that the microring optical resonator could real-time monitor the reaction between biological molecules, the resonator could be used in the quantitative detection and biological sensing.
- Silicon microring optical resonator,
- Biosensor,
- Microfluidics,
- Evanescent wave,
- Immunoglobulin G
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