Citation: BAI Ru-Yan, ZHANG Kun-Lei, LI De-Lei, ZHANG Xi, LIU Ting-Zhi, LIU Yi, HU Rong, YANG Yun-Hui. Preparation of Carcinoembryonic Antigen Immunosensor Based on Au Nanoparticles Loaded-Metal-Organic Frameworks[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(1): 48-55. doi: 10.11895/j.issn.0253-3820.160611 shu

Preparation of Carcinoembryonic Antigen Immunosensor Based on Au Nanoparticles Loaded-Metal-Organic Frameworks

College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China

Received Date: 17 August 2016
Revised Date: 1 November 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.21465026,21165023)

A novel and simple electrochemical immunoassay for carcino-embryonic antigen (CEA) was developed using Au nanoparticles loaded-metal-organic frameworks (AuNPs/Cu-TPA) as signal unit and electrochemical reduction of graphene oxide on the surface of electrode to capture CEA antibody (Ab1). MOFs contain large amounts of Cu²⁺ ions, which can be used as stable electrochemical signals to achieve the detection of CEA. This new class of signal probe differs from traditional probe because it does not require pre-treatment and acid treatment, and easy to load noble metal for the immobilization of antibody, which will greatly simplify the detection steps and reduce the detection time. This immunosensor has good sensitivity and ease to operate. Under the optimized experimental conditions, the proposed sensing strategy provides a linear dynamic range from 0.1 ng/mL to 80 ng/mL with a detection limit of 0.03 ng/mL and linear correlation coefficient of 0.9887. The developed CEA immunosensor can be used to detect CEA in real sample.
- Carcino-embryonic antigen,
- Metal organic framework,
- Electrochemical immunosensor
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