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Citation: WANG Yu, REN Shu-Yue, JIANG Hui-Cong, BAI Jia-Lei, PENG Yuan, NING Bao-An, GAO Zhi-Xian. Upconversion Nanoparticles-based Immunochromatographic Strip for Rapid Determination of Diethylstilbestrol[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(1): 35-41. doi: 10.11895/j.issn.0253-3820.160610 shu

Upconversion Nanoparticles-based Immunochromatographic Strip for Rapid Determination of Diethylstilbestrol

1.
Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environment Medicine, Tianjin 300050, China;
2.
School of Public Health, Zhengzhou University, Zhengzhou 450001, China

Received Date: 17 August 2016
Revised Date: 20 October 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.21477162,AWS15J006)

The upconversion nanoparticles (UCNPs) NaYF₄∶Yb,Er were synthesized by the solvent thermal method. Fluorescence probe was prepared by coupling the anti-diethylstilbestrol (DES) monoclonal antibody with UCNPs. The DES-labeled bovine serum albumin (BSA) conjugates and goat anti-mouse antibodies were sprayed onto the nitrocellulose membrane as the test line and the control line, respectively. The resultant fluorescence probes were introduced into the immunochromatographic strip for rapid determination of DES. The UCNPs-based immunochromatographic strips for determination of DES exhibited a good linear range from 25 ng/mL to 10000 ng/mL (y=0.43927x-0.57647, R²=0.996), with a limit of detection of 20.84 ng/mL. The detection time of the proposed UCNPs based immunochromatographic strips for each sample was only 15 min. The RSDs of the intra and inter assay were less than 10%. The strip showed excellent specificity to structural and functional analogues, and the average RSD of detection value after storage at 37℃ for 7 days was about 15%. The recovery experiment showed that the average recoveries of DES at 5 spiked concentration levels were 90.1%-115.2%, and the RSDs were below 5%. These results were consistent with that of HPLC.
- Upconversion nanoparticles,
- Immunochromatographic strip,
- Diethylstilbestrol,
- Quantitative determination
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