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Citation: LIANG Ju-Chao, QU Yin, YU Miao-Miao, XU Ling-Ling, LIU Ya-Jie, SUN Zhan-Xue, CHEN Huan-Wen. Rapid Analysis of Acidithiobacillus Ferrooxidans Metabolites by Ambient Corona Discharge Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(11): 1721-1727. doi: 10.11895/j.issn.0253-3820.160531 shu

Rapid Analysis of Acidithiobacillus Ferrooxidans Metabolites by Ambient Corona Discharge Ionization Mass Spectrometry

1.
East China Univesity of Technology, Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, Nanchang 330013, China;
2.
East China Univesity of Technology, State Key Laboratory Breeding Base of Nuclear Resources and Environment, Nanchang 330013, China

Received Date: 12 July 2016
Revised Date: 2 September 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 81460327), Program for Changjiang Scholars and Innovation Research Team in Universities (PCSIRT) (No. IRT13054), Science and technology support program of Jiangxi Province (Nos. 20152ACB21013, 20152ACG70014)

A method for rapid determination of acidithiobacillus ferrooxidans (At.f) metabolites by ambient corona discharge ionization mass spectrometry was established. Three injection modes were applied to study the effects such as headspace sampling, interface sampling and neutral desorption sampling. Under the optimized experimental conditions, the metabolites, such as small molecule esters and amines were detected by ambient corona discharge ionization mass spectrometry and confirmed using tandem mass spectrometry (MS/MS). Further principal component analysis (PCA) and cluster analysis (CA) of the mass spectrometric results allowed a confident discrimination of different bacterial samples. Ambient corona discharge ionization mass spectrometry has the advantages of no sample pretreatment, convenient operation, high sensitivity and high analytical speed, which will be an attractive method to rapidly identification of microorganisms.
- Acidithiobacillus ferrooxidans,
- Ambient corona discharge ionization,
- Metabolites,
- Mass spectrometry
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