Citation: FU Xin, GU Dan-Yu, ZHAO Sheng-Dong, WEN Shi-Tong, ZHANG He. Study on Biosensor for Ag⁺ and Cysteine Quantification Based on Magnetic Nanoparticles and Intermolecular Split G-Quardruplex-Hemin DNAzymes[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(10): 1487-1494. doi: 10.11895/j.issn.0253-3820.160508 shu

Study on Biosensor for Ag⁺ and Cysteine Quantification Based on Magnetic Nanoparticles and Intermolecular Split G-Quardruplex-Hemin DNAzymes

School of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, China

Received Date: 9 April 2016
Revised Date: 16 August 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21005067) and the Natural Science Foundation of Hunan Province, China (Nos.2015JJ2039, 14JJ3133)

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Based on intermolecular split G-quardruplex-hemin DNAzymes, a biosensor for detection of silver ions and cysteine was developed with magnetic nanoparticles (MNPs) as carrier to immobilize the DNA probes. Since Ag⁺ chelates guanine bases at the binding sites which are involved in G-quadruplex formation, the presence of Ag⁺ may inhibit G-quartets connected by Hoogsteen-type base pairing and disrupt G-quadruplexes structures, which decreases the peroxidase activity of G-quadruplex-hemin DNAzymes that efficiently catalyze H₂O₂-mediated reactions, such as the oxidation of ABTS (2,2'-azinobis(3-ethylbenzothiozoline)-6-sulfonic acid) by H₂O₂. Moreover, in the presence of L-cysteine, it was used as a competitor by the strongly Ag-S to release Ag⁺ from G-rich oligonucleotides, promoting the reformation of G-quadruplexes and increasing the peroxidase activity, which catalyzes the ABTS-H₂O₂ reaction system. In this experiment, the efficient separation from real sample was achieved using magnetic nanoparticles as a solid phase carrier to effectively increase the detection sensitivity and decrease the background signal. Under the optimum conditions, a high linear relationship between the UV absorbance and the Ag⁺ concentration was established in the range of 0.5-100 nmol/L with a detection limit of 0.2 nmol/L. The calibration curve of cysteine was identified in the range from 0.1 to 80 nmol/L and the detection limit was as low as 0.04 nmol/L.
- Magnetic nanoparticles,
- Intermolecular split G-quardruplex,
- Colorimetric assay,
- Silver ions,
- Cysteine
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沈阳化工大学材料科学与工程学院沈阳 110142

Study on Biosensor for Ag+ and Cysteine Quantification Based on Magnetic Nanoparticles and Intermolecular Split G-Quardruplex-Hemin DNAzymes

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通讯作者: 陈斌, bchen63@163.com

Study on Biosensor for Ag⁺ and Cysteine Quantification Based on Magnetic Nanoparticles and Intermolecular Split G-Quardruplex-Hemin DNAzymes