Home

Citation: SHEN Hai-Ying, WANG Xiao-Qian, WANG Yun-Yun, QU Feng. Immunoassay for Alpha Fetal Protein in Serum Based on Quartz Capillary Materials by Gray Level Analysis[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(1): 83-88. doi: 10.11895/j.issn.0253-3820.160417 shu

Immunoassay for Alpha Fetal Protein in Serum Based on Quartz Capillary Materials by Gray Level Analysis

1.
Key Laboratory of Convergence Medical Engineering System and Healthcare Technology, the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijng 100081, China;
2.
CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China;
3.
Department of Clinical Laboratory in South Building, General Hospital of PLA, Beijing 100853, China

Received Date: 1 June 2016
Revised Date: 20 September 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21675012)

A fused quartz capillary was taken as the carrier for the immunoassay of the tumor marker alpha fetal protein (AFP) as a model protein. The capture antibody of AFP was immobilized in capillary. Based on a sandwich immunoassay format, the antigen and corresponding horseradish peroxidase (HRP) labeled antibodies were introduced into the capillary for online incubation. After the injection of the chemiluminescence substrate, the chemiluminescence signals were captured by the CCD camera and changed into figures. The results could be read by gray level analysis of the figures. When the capture antibody was diluted 100 times, the enzyme labeled antibody was diluted 200 times, and the exposure time was 20 s, the detection range of AFP was 3.1-50 ng/mL and the critical concentrations of 20 ng/mL clinical applications were covered. The limit of detection was 2.7 ng/mL. Twenty clinical samples were tested with fine accuracy. The recoveries of the spiked samples were between 96.0% and 106.7%. In the assay, the sample consumption was low and the experimental material was simple and cheap. The method does not require large instruments, and is appropriate for clinical application
- Quartz capillary,
- Alpha fetal protein,
- Immunoassay,
- Gray level analysis
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
1. [1]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
2. [2]
  Zhuomin Zhang , Hanbing Huang , Liangqiu Lin , Jingsong Liu , Gongke Li . Course Construction of Instrumental Analysis Experiment: Surface-Enhanced Raman Spectroscopy for Rapid Detection of Edible Pigments. University Chemistry, 2024, 39(2): 133-139. doi: 10.3866/PKU.DXHX202308034
3. [3]
  Zhenli Sun , Ning Wang , Kexin Lin , Qin Dai , Yufei Zhou , Dandan Cao , Yanfeng Dang . Visual Analysis of Hotspots and Development Trends in Analytical Chemistry Education Reform. University Chemistry, 2024, 39(11): 57-64. doi: 10.12461/PKU.DXHX202403095
4. [4]
  Zhening Lou , Quanxing Mao , Xiaogeng Feng , Lei Zhang , Xu Xu , Yuyang Zhang , Xueyan Liu , Hongling Kang , Dongyang Feng , Yongku Li . Practice of Implementing Blended Teaching in Shared Analytical Chemistry Course. University Chemistry, 2024, 39(2): 263-269. doi: 10.3866/PKU.DXHX202308089
5. [5]
  Yan Zhang , Ping Wang , Tiebo Xiao , Futing Zi , Yunlong Chen . Measures for Ideological and Political Construction in Analytical Chemistry Curriculum. University Chemistry, 2024, 39(4): 255-260. doi: 10.3866/PKU.DXHX202401017
6. [6]
  Xiaofei Zhou , Yu-Qing Cao , Feng Zhu , Li Qi , Linhai Liu , Ni Yan , Zhiqiang Zhu . Missions and Challenges of Instrumental Analysis Course in the New Era. University Chemistry, 2024, 39(6): 174-180. doi: 10.3866/PKU.DXHX202310058
7. [7]
  . . Chinese Journal of Inorganic Chemistry, 2024, 40(12): 0-0.
8. [8]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
9. [9]
  Lijun Dong , Pengcheng Du , Guangnong Lu , Wei Wang . Exploration and Practice of Independent Design Experiments in Inorganic and Analytical Chemistry: A Case Study of “Preparation and Composition Analysis of Tetraammine Copper(II) Sulfate”. University Chemistry, 2024, 39(4): 361-366. doi: 10.3866/PKU.DXHX202310041
10. [10]
  Jingyi Chen , Fu Liu , Tiejun Zhu , Kui Cheng . Practice of Integrating Ideological and Political Education into Raman Spectroscopy Analysis Experiment Course. University Chemistry, 2024, 39(2): 140-146. doi: 10.3866/PKU.DXHX202310111
11. [11]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
12. [12]
  Liuchuang Zhao , Wenbo Chen , Leqian Hu . Discussion on Improvement of Teaching Contents about Common Evaluation Parameters in Analytical Chemistry. University Chemistry, 2024, 39(2): 379-391. doi: 10.3866/PKU.DXHX202308079
13. [13]
  Wenyan Dan , Weijie Li , Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060
14. [14]
  Guangming Yang , Yunhui Long . Design and Implementation of Analytical Chemistry Curriculum Based on the Learning Community of Teachers and Students. University Chemistry, 2024, 39(3): 132-137. doi: 10.3866/PKU.DXHX202309089
15. [15]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Application of Comparative Pedagogy in Instrumental Analysis Experiment Teaching. University Chemistry, 2024, 39(3): 266-273. doi: 10.3866/PKU.DXHX202309068
16. [16]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
17. [17]
  Qiang Xu , Rong Zhang , Liyan Zhang , Jinxuan Liu , Shuo Wu , Rongwen Lv . Exploration and Practice of Ideological and Political Education Construction in the Course of Practical Instrument Analysis Theory. University Chemistry, 2024, 39(6): 132-136. doi: 10.3866/PKU.DXHX202311018
18. [18]
  Tian Li , Liping Zhang , Ling Liu , Ruifang Li , Longfei Mao , Hui Yang . Reform and Practice of Analytical Chemistry Teaching under the Guidance of Course Ideology and Politics. University Chemistry, 2024, 39(6): 189-194. doi: 10.3866/PKU.DXHX202310014
19. [19]
  Tianlong Zhang , Jiajun Zhou , Hongsheng Tang , Xiaohui Ning , Yan Li , Hua Li . Virtual Simulation Experiment for Laser-Induced Breakdown Spectroscopy (LIBS) Analysis. University Chemistry, 2024, 39(6): 295-302. doi: 10.3866/PKU.DXHX202312049
20. [20]
  Fan Yu , Aihua Li , Yun Liu , Tianrong Zhu , Liang Wang , Junhui Xu , Yazhen Wang . Exploration and Practice in Developing a Premier Course in Inorganic and Analytical Chemistry. University Chemistry, 2024, 39(8): 36-43. doi: 10.3866/PKU.DXHX202312037

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(465)
HTML views(32)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return