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Citation: SHEN Hai-Ying, WANG Xiao-Qian, WANG Yun-Yun, QU Feng. Immunoassay for Alpha Fetal Protein in Serum Based on Quartz Capillary Materials by Gray Level Analysis[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(1): 83-88. doi: 10.11895/j.issn.0253-3820.160417 shu

Immunoassay for Alpha Fetal Protein in Serum Based on Quartz Capillary Materials by Gray Level Analysis

1.
Key Laboratory of Convergence Medical Engineering System and Healthcare Technology, the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijng 100081, China;
2.
CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China;
3.
Department of Clinical Laboratory in South Building, General Hospital of PLA, Beijing 100853, China

Received Date: 1 June 2016
Revised Date: 20 September 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21675012)

A fused quartz capillary was taken as the carrier for the immunoassay of the tumor marker alpha fetal protein (AFP) as a model protein. The capture antibody of AFP was immobilized in capillary. Based on a sandwich immunoassay format, the antigen and corresponding horseradish peroxidase (HRP) labeled antibodies were introduced into the capillary for online incubation. After the injection of the chemiluminescence substrate, the chemiluminescence signals were captured by the CCD camera and changed into figures. The results could be read by gray level analysis of the figures. When the capture antibody was diluted 100 times, the enzyme labeled antibody was diluted 200 times, and the exposure time was 20 s, the detection range of AFP was 3.1-50 ng/mL and the critical concentrations of 20 ng/mL clinical applications were covered. The limit of detection was 2.7 ng/mL. Twenty clinical samples were tested with fine accuracy. The recoveries of the spiked samples were between 96.0% and 106.7%. In the assay, the sample consumption was low and the experimental material was simple and cheap. The method does not require large instruments, and is appropriate for clinical application
- Quartz capillary,
- Alpha fetal protein,
- Immunoassay,
- Gray level analysis
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