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Citation: GAO Xue-Jin, LI Jiao, LYU Xin-Yu, WANG Pu. Research on Detection Method of Enzyme Injection Glucose Biosensors Based on Temperature[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(1): 97-103. doi: 10.11895/j.issn.0253-3820.160373 shu

Research on Detection Method of Enzyme Injection Glucose Biosensors Based on Temperature

College of Electronic and Control Engineering, Beijing University of Technology, Engineering Research Center of Digital Community, Ministry of Education, Beijing Laboratory For Urban Mass Transit, Beijing key Laboratory of Computational Intelligence and Intelligent System, Beijing 100124, China

Received Date: 17 May 2016
Revised Date: 12 September 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.61174109,61640312)

To avoid the inaccuracy measurement caused by the different temperature between calibration solution and test solution in actual use of enzyme injection glucose biosensor, a detection method was established for enzyme injection glucose biosensors based on temperature. Firstly, the concentration detection model of the enzyme injection glucose biosensor is established according to enzymatic reaction kinetics. Then, the relationship between the temperature and the unknown parameters in the dynamic model of concentration detection is built using Arrhenius equation, and this relationship is substituted into the original detection dynamics model to establish the new model based on temperature. In this model, the current initial slope of the enzymatic reaction and the temperature are the inputs, and the concentration of glucose is output. Based on this new model, a detection method which is derived by the temperature of the mixture and the slope of the initial current is proposed. The new detection method can not only reduce the impact of temperature difference to improve detection accuracy, but also avoid the removal procedure of sampling probe causing by the calibration before each measurement, and it is more conducive to online use. A comparison was made by detecting the glucose solution with a concentration of 1.5 mg/mL, 2.5 mg/mL at the temperature of 25, 30, and 42℃ using both the original detection method and the new detection method based on temperature. The result shows that the recovery rate of the new method is more than 95.0%, significantly better than that of the original detection method.
- Temperature,
- Glucose,
- Biosensor
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