Citation: ZONG Chun-Guang, LI Shuang, XU Ji-Lin, LI Yan-Rong, ZHONG Ying-Ying, YU Xue-Yun, ZHOU Cheng-Xu, YAN Xiao-Jun. ¹³C Stable Isotope Labeled Quantitative Analysis of Photosynthesis Lipid in Diatom[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(11): 1698-1706. doi: 10.11895/j.issn.0253-3820.160354 shu

¹³C Stable Isotope Labeled Quantitative Analysis of Photosynthesis Lipid in Diatom

1.
Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China;
2.
Ningbo Entry-Exit Inspection and Quarantine Office, Ningbo 315000, China

Received Date: 6 May 2016
Revised Date: 8 September 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No.31172448)

The diatom Nitzschia closterium f. minutissima was selected as the object to study the ¹³C labeled extent and speed of photosynthesis lipids by ultra high pressure liquid chromatography coupled with quadrupole orbitrap high resolution mass spectrometry. The results showed that all four types of lipid were labeled clearly by ¹³C within 10 days in the stationary phase. The total labeled amount by ¹³C of digalactosyl diacylglycerol (DGDG), phosphatidylglycerol (PG) and sulfoquinovosyl diacylglycerol (SQDG) increased from entering the stationary phase to the eighth day, but declined later. And the total labeled amount of DGDG, PG and SQDG reached the maximum of (173±24) ng/mg, (473±41) ng/mg and (1224±21) ng/mg on the eighth day with the labeled rate of 56.3%, 38.4% and 62.6%, respectively. The total labeled amount by ¹³C of monogalactosyl diacylglycerol (MGDG) showed a continued upward trend during the 10 days, and reached (956±51) ng/mg on the tenth day, with marked rate of 87.3%. It was suggested that the synthesized time of different lipid was different. Therefore, for studying assimilation mechanism of microalgae lipids by marine organisms, the lipid content that labeled by ¹³C should reach the maximum. So if for DGDG, PG and SQDG, we should choose the diatom labeled 8 days by ¹³C to feed the marine organisms. Meanwhile if for MGDG, we should choose the diatom labeled 10 days by ¹³C to feed the marine organisms.
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

13C Stable Isotope Labeled Quantitative Analysis of Photosynthesis Lipid in Diatom

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通讯作者: 陈斌, bchen63@163.com

¹³C Stable Isotope Labeled Quantitative Analysis of Photosynthesis Lipid in Diatom