Citation: HUANG Xin, LI Shuai-Ping, ZHANG Yong, LIU Shu-Ying. Simultaneous Determination of Bioamine and Amino Acid Neurochemicals in Rat Hippocampus and Cerebral Cortex by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(11): 1652-1658. doi: 10.11895/j.issn.0253-3820.160338 shu

Simultaneous Determination of Bioamine and Amino Acid Neurochemicals in Rat Hippocampus and Cerebral Cortex by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
Changchun University of Chinese Medicine, Jilin Ginseng Academy, Changchun 130117, China;
2.
Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received Date: 27 April 2016
Revised Date: 8 June 2016

An ultra performance liquid chromatography-tandem mass (UPLC-MS/MS) spectrometry method was established for determination of the contents of bioamine and amino acid neurochemicals in hippocampus and cerebral cortex of rat. Hypercarb column (100 mm×2.1 mm, 5 μm) was used for the sample separation with 0.1% formic acid-methanol as the mobile phase under gradient elution. The neurochemicals were detected by MS/MS using ESI ion source under positive ionization mode with MRM scan. Both quantification and confirmation ions were chosen for each 6 bioamine and 11 amino acid neurochemicals. The influence of two different processing methods on the contents of neurochemicals in brain tissues was compared. Total 17 neurochemicals were simultaneously detected in 10 min. The calibration curve was with a good linear relationship. The intra-day and inter-day precision, average recovery and repeatability could meet the analysis requirement. This UPLC-MS/MS method shows excellent selectivity, accuracy, high sensitivity, specificity and good repeatability, and is suitable for the separation and quantization of bioamine and amino acid neurochemicals in brain tissue.
- Ultra performance liquid chromatography-tandem mass spectrometry,
- Bioamine neurochemicals,
- Amino acids neurochemicals,
- Brain tissue
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沈阳化工大学材料科学与工程学院沈阳 110142