Citation: YANG Chun, ZHOU Xing-Lu, LIU Ya-Ru, ZHANG Yan, WANG Jian, TIAN Li-Li, YAN Ya-Nan. Extensive Imprinting Adaptability of Polyacrylamide-based Amphoteric Cryogels Against Protein Molecules[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(9): 1322-1327. doi: 10.11895/j.issn.0253-3820.160292 shu

Extensive Imprinting Adaptability of Polyacrylamide-based Amphoteric Cryogels Against Protein Molecules

College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China

Received Date: 14 April 2016
Revised Date: 21 June 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21375115), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Figures(4)

Lysozyme, pepsin, ovalbumin, hemoglobin and γ-globumin were chosen as templates to investigate the imprinting capability of amphoteric polyacrylamide cryogels. Prepolymerizing solutions contained acrylic acid and allyl amine, as well as acrylamide and N,N'-methylenebisacrylamide as the functional monomers. As a result there were both acidic and basic functional groups in the polymers, facilitating effective interactions with likewise amphoteric proteins. The proteins differ greatly and cover wide scopes of molecular weights and isoelectric points. Regardless of the values of the molecular weights and isoelectric points, all the templates gave higher retentions on the MIP tubes than on the NIP tube. The MIP of lysozyme indicated the highest imprinting factor of 7.0, and that of γ-globumin showed the lowest, 2.0. The values of other proteins were intervenient. Conclusively the amphoteric polyacrylamide cryogels were suitable imprinting materials for various proteins, and could potentially be used for protein recognition, purification, and depletion.
- Molecularly imprinted polymer,
- Amphoteric cryogel,
- Protein,
- Imprinting factor
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沈阳化工大学材料科学与工程学院沈阳 110142

Extensive Imprinting Adaptability of Polyacrylamide-based Amphoteric Cryogels Against Protein Molecules

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com