Citation: ZHANG Qian, WANG Song-Meng, CHU Li-Qiang. Immobilization and Hybridization of Oligo-adenine Sequence Containing DNA Probes on Gold Nanoparticles[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(1): 1-6. doi: 10.11895/j.issn.0253-3820.160290 shu

Immobilization and Hybridization of Oligo-adenine Sequence Containing DNA Probes on Gold Nanoparticles

College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, China

Received Date: 13 April 2016
Revised Date: 2 November 2016

Oligo-adenine sequence (OAS) containing DNA probes (DNA_probe) was immobilized on gold nanoparticles (AuNPs) with various surface densities via the strong interaction between OAS sequence and gold surface, and the influences of different conditions (e.g., length of OAS, size of AuNPs, concentration of NaCl, etc.) on the immobilization of DNA_probe and hybridization performance of the resulting nanoparticle probe (Au-probe) were investigated. Transmission electron microscopy (TEM), ultraviolet-visible absorption spectroscopy (UV-Vis) and nanoparticle size analyzer were utilized to characterize the morphology and size of AuNPs and Au-probe, as well as the DNA_probe immobilization on AuNPs surface and their hybridization with DNA target(DNA_target). The results showed that, when the number of adenine in an OAS sequence increased from 10, to 30 and 50, the amount of the immobilized DNA_probe decreased as expected. The optimal NaCl concentrations for the hybridization were 300 mmol/L for 10.2-nm AuNPs, and 25 mmol/L for 24.3-nm AuNPs, respectively. With increasing size of AuNPs, the amount of both immobilized DNA_probe and hybridized DNA_target decreased.
- Oligo-adenine sequence,
- Gold nanoparticles,
- Deoxyribonucleic acid immobilization,
- Deoxyribonucleic acid detection,
- Hybridization performance
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