Citation: SUN Jia-Ming, TIAN Lin-Lin, HE Zhong-Mei, MENG Ling-Wen, SUN Hui, KAN Jun-Ming, ZHANG Hui. Rapid Screening of Active Components of Lepidium Meyenii Promoting Leydig Cell Proliferation by Electrospray Ionization Mass Spectrometry and Chemometrics Methods[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(11): 1735-1741. doi: 10.11895/j.issn.0253-3820.160232 shu

Rapid Screening of Active Components of Lepidium Meyenii Promoting Leydig Cell Proliferation by Electrospray Ionization Mass Spectrometry and Chemometrics Methods

1.
Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China;
2.
College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130117, China;
3.
Graduate school, Changchun University of Chinese Medicine, Changchun 130117, China

Received Date: 26 March 2016
Revised Date: 12 July 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No.31570347)

The low-polarity constituents from Lepidium meyenii in Yunnan and Peru were detected by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The effects of 10 samples of Lepidium meyenii on proliferation of rat leydig cells were measured. After the process of MS data using principal component analysis (PCA) methods, the samples from Yunnan and Peru were distinguished effectively. Subsequently, the grey relational degree analysis (GRA) and partial least squares analysis (PLS) were employed to reveal the correlation between common peaks of ESI fingerprint of Lepidium meyenii from different geographical origins and proliferation activity of rat leydig cells. The results suggested that most low-polarity constituents from Lepidium meyenii had good activities on proliferation of rat leydig cells, in which N-benzylhexadecanamide and N-benzy-(9Z,12Z,15Z)-octadecatrienamide exhibited the highest activities. In addition, the proposed procedure could also be used as a simple, quick reference method to screen the active components promoting Leydig cell proliferation in traditional Chinese medicine.
- Lepidium meyenii,
- Different geographical origin,
- Electrospray ionization tandem mass spectrometry,
- Chemometrics method,
- Promoting leydig cell proliferation
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