Citation: LIN He, PI Zi-Feng, MEN Li-Hui, CHEN Wei-Jia, LIU Zhi-Qiang, LIU Zhong-Ying. Untargeted Metabonomic Study of Compatibility of Panax Ginseng and Veratrum Nigrum in Spleen qi Deficiency Rat[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(11): 1755-1762. doi: 10.11895/j.issn.0253-3820.160224 shu

Untargeted Metabonomic Study of Compatibility of Panax Ginseng and Veratrum Nigrum in Spleen qi Deficiency Rat

1.
College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China;
2.
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China;
3.
Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received Date: 24 March 2016
Revised Date: 12 August 2016

Fund Project: This work was supported by the National Basic Research Program of China (973 Program) (Nos. 2011CB505300, 2011CB505305)

A urinary untargeted metabonomics method based on UPLC/Q-TOF-MS was established to investigate the mechanism of compatibility of Panax ginseng and Veratrum nigrum in Spleen qi deficiency rat. Spleen qi deficiency rat model was established, then the exhaustive swimming time and the content of white blood cells, red blood cell and hemoglobin in the blood was detected after administration for 15 days. The results showed that Panax ginseng could increase the exhaustion swimming time (p<0.01) and the content of white blood cells, red blood cell and hemoglobin in the blood (p<0.05, p<0.01) of spleen qi deficiency rats, and Veratrum nigrum or compatible application had no significant effect index mentioned above (p>0.05). The urinary metabolic profiling was analyzed by using UPLC/Q-TOF-MS and the obtained data were analyzed by PCA. The score of PCA showed that there was significant difference among the metabolic profile of different groups. According to the results of OPLS-DA and the databases, fifteen potential biomarkers were identified as the therapeutic effect of Panax ginseng, and seven of them and their pathways were considered as reducing the therapeutic effect of Panax ginseng by co-administration with Veratrum nigrum. The results indicated that the effect of Panax ginseng on promoting energy metabolism, regulating immune and antioxidation in the spleen qi deficiency rats were decreased after the compatibility of Panax ginseng and Veratrum nigrum.
- Panax ginseng,
- Veratrum nigrum,
- Incompatibility,
- Spleen qi deficiency,
- Untargeted metabonomics
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沈阳化工大学材料科学与工程学院沈阳 110142