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Citation: ZHANG Lian-Ming, HUANG Xiao-Yun, ZHANG Ying-Ming, LI Jian-Ping, ZHANG Lan, ZENG Ying. A Novel Molecularly Imprinted Fluorescence Test Strip for Detection of Cimaterol[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(10): 1477-1481. doi: 10.11895/j.issn.0253-3820.160204 shu

A Novel Molecularly Imprinted Fluorescence Test Strip for Detection of Cimaterol

1.
College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, China;
2.
Guangxi Zhuang Autonomous Region Center for Analysis and Test Research, Nanning 530000, China;
3.
College of Material and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610000, China

Received Date: 19 March 2016
Revised Date: 20 August 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21375031), the Natural Science Foundation of Guangxi Province, China (Nos. 2015GXNSFFA139005, 2015GXNSFAA139029) and Guangxi Science and Technology Major Project, China (No. 1222003-2-2)

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A novel test strip for rapid detection of cimaterol was prepared by combining highly specific selectivity of molecularly imprinted technology with convenience and rapid response performance of test strip. The cimaterol molecularly imprinted polymers (MIPs) were prepared through chemical polymerization method, and the nitrocellulose membrane was immersed into MIPs solution. After that, the template molecules were eluted and then a clipping step was conducted for obtaining the test strip. After re-adsorption of cimaterol, fluorescent dye Eosin Y was added onto the surface reaction zone on the strip. Qualitative and quantitative analysis could be realized via determining fluorescence quenching in the reaction zone. The experimental results showed that the quenching of fluorescence had a good linear relationship with the cimaterol concentration ranging from 0.01 to 100 μg/mL with a limit of detection of 0.01 μg/mL. The molecular imprinting test strips provided a convenient way in practical application especially for the quick detection of pork and fodder sample on site.
- Test strip,
- Molecularly imprinted,
- Cimaterol,
- Fluorescence,
- Eosin Y
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