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Citation: WANG Gao-Hong, HUANG Xin-Yi, DUAN Wen-Da, QUAN Kai-Jun, WANG Bing-Peng, FAN Rui-Na, DI Duo-Long. Advances in Technology of Countercurrent Chromatography for Separation of Protein and Peptide[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(10): 1600-1608. doi: 10.11895/j.issn.0253-3820.160152 shu

Advances in Technology of Countercurrent Chromatography for Separation of Protein and Peptide

WANG Gao-Hong ^1,2, ,
HUANG Xin-Yi ¹ ,
DUAN Wen-Da ^1,2 ,
QUAN Kai-Jun ^1,2 ,
WANG Bing-Peng ^1,3 ,
FAN Rui-Na ^1,3 ,
DI Duo-Long ¹

1.
Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
2.
Graduate University of Chinese Academy of Sciences, Beijing 100039, China;
3.
Pharmaceutical college, Gansu University of Chinese Medicine, Lanzhou 730000, China

Received Date: 3 March 2016
Revised Date: 3 August 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21175142, 21505144) and the Natural Science Foundation of Gansu Province, China (No. .1506RJZA283)

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Countercurrent chromatography is a kind of continuous and effective liquid-liquid partition chromatography with many unique characteristics such as large load capacity, no irreversible adsorption, high recovery rate, low risk of sample denaturation and so on, which has irreplaceable advantages in separation of protein and peptide. This review presents the advances of several kinds of new technology of countercurrent chromatography in the separation of protein and peptide. The developing prospect of this field is also discussed.
- Countercurrent chromatography,
- Protein,
- Peptide,
- Review
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