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Citation: ZHANG Xin Ying, ZHENG Ling Na, WANG Hai Long, SHI Jun Wen, FENG Wei Yue, LI Liang, WANG Meng. Elemental Bio-imaging of Biological Samples by Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(11): 1646-1651. doi: 10.11895/j.issn.0253-3820.160109 shu

Elemental Bio-imaging of Biological Samples by Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry

1.
School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan 430205, China;
2.
Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China

Received Date: 19 February 2016
Revised Date: 11 April 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21775136, U1432241, 11505194)

Trace elements play a very important role in biological organism and closely related to many diseases. The new analytical methods are urgently needed for in-situ determination of trace elements in biological tissues or single cells. A method based on laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was developed for such in-situ analysis. Line ablation mode was chosen and the output energy of the laser was also optimized at a low fluence of less than 1 J/cm². The two kinds of elemental bio imaging of both a coronal section from mouse brain and single cells exposed to gold nanoparticles were obtained by the developed method. Because of the unique characteristics, such as good spatial resolution, excellent detection limit, and reasonable running cost, LA-ICP-MS will be applied widely and become a useful tool in biomedical research in the future.
- Inductively coupled plasma-mass spectrometry,
- Laser ablation,
- Elemental bio-imaging,
- Single cell analysis,
- Mouse brain sections,
- Gold nanoparticles
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