Citation: LIU Xing-Fen, WANG Ya-Teng, HUA Xiao-Xiao, HUANG Yan-Qin, FENG Xiao-Miao, FAN Qu-Li, HUANG Wei. Rapid Detection of Lead Ion (Ⅱ) Based on Cationic Conjugated Polymer and Aptamer[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(7): 1092-1098. doi: 10.11895/j.issn.0253-3820.160010 shu

Rapid Detection of Lead Ion (Ⅱ) Based on Cationic Conjugated Polymer and Aptamer

1.
Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing 210023, China;
2.
Key Laboratory of Flexible Electronics & Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University (Nanjing Tech), Nanjing 211816, China

Received Date: 5 January 2016
Revised Date: 19 April 2016

Fund Project: This work was supported by the State Key Development Program for Basic Research of China (Nos. 2012CB933301, 2012CB723402), and the National Natural Science Foundation of China (Nos. 21005040, 51173080)

Figures(5)

A sensitive method for Pb²⁺ detection based on a cationic conjugated polymer and aptamer was established. By selecting a more specific aptamer probe, the interference of Pb²⁺ detection from Hg²⁺ was eliminated remarkably. The probe for Pb²⁺ recognition and combination is a single stranded oligonucleotide labeled with fluorescein (TBAA, 5'-6-FAM-GGAAGGTGTGGAAGG-3'). When combining with Pb²⁺ with high specificity, the random coiled probe changed to a G-quadruplex with higher charge density, which enhanced the electrostatic interactions between the oligonucleotide and cationic conjugated polymer, thus the two fluorophores were in more close proximity, leading to a significantly increased fluorescence resonance energy transfer (FRET) signal. However, other non-target metal ions produced much lower FRET signals because they could not combine with the probe and thus quenched the fluorescence of the conjugated polymer and fluorescein. This method was rapid, highly specific and sensitive, and the common metal ions including Li⁺, Na⁺, K+, Ca²⁺, Mg²⁺, Ba²⁺, Fe²⁺, Co²⁺, Ni²⁺, Cu²⁺, Zn²⁺, Cd²⁺ and Hg²⁺ exhibited no influence on the detection of Pb²⁺. The limit of detection for Pb²⁺ in lake water was estimated to be 1 nmol/L (3σ), which was far below the maximum permitted level of Pb²⁺ in drink water regulated by the national standard for drinking water quality. Therefore, this FRET-based method provides a new simple, rapid, and efficient method for detection of Pb²⁺ in various source of water.
- Cationic conjugated polymer,
- Aptamer,
- Lead ion (Ⅱ),
- Fluorescence resonance energy transfer
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