Citation: HUANG Zheng, ZHANG Hong-Yi. Determination of Mercapto Compounds by Pre-column Derivatization-Acid-induced Transient Isotachophoretic Stacking Capillary Electrophoresis[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(7): 1065-1070. doi: 10.11895/j.issn.0253-3820.151021 shu

Determination of Mercapto Compounds by Pre-column Derivatization-Acid-induced Transient Isotachophoretic Stacking Capillary Electrophoresis

College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China

Received Date: 28 December 2015
Revised Date: 22 April 2016

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A new method was developed for the determination of mercapto compounds by pre-column derivatization combined with acid-induced transient isotachophoretic stacking capillary electrophoresis. Mercapto compounds were derivatized with 2-chloro-1-methylpyridinium iodide (CMPI) in Tris-HCl buffer solution at pH 8.15 at room temperature. The derivatized mercapto compounds were introduced into the capillary filled up with 40 mmol/L formate buffer (pH=3.96), followed by injection of diluted sulfuric acid. Upon application of voltage at normal polarity, the derivatized mercapto compounds were preconcentrated due to transient isotachophoresis stacking electrophoresis with H⁺ as the leading ion and Tris⁺ as the terminating ion. The detection wavelength was set at 312 nm. Cysteine and sodium thioglycolate were chosen as the models of mercapto compounds to be detected. Various experimental factors influencing transient isotachophoresis stacking were investigated. Under the optimal conditions, the linear relation between peak area and concentration was obtained in the range from 10 mg/L to 100 mg/L for cysteine, and from 4 mg/L to 100 mg/L for sodium thioglycolate. The LODs were 0.2 mg/L and 0.4 mg/L for cysteine and sodium thioglycolate, respectively. The developed method was successfully applied to the determination of thioglycolate in depilatory cream samples.
- Mercapto compounds,
- Cysteine,
- Sodium thioglycolate,
- Pre-column derivation,
- On-column enrichment,
- Capillary electrophoresis
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