Citation: PENG Qi-Sheng, LI Guang-Pu, SUN Wan-Chun, YANG Jing-Bo, QUAN Gui-Hua, LIU Ning. Analysis of ISG15-Modified Proteins from A549 Cells in Response to Influenza Virus Infection by Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(6): 850-856. doi: 10.11895/j.issn.0253-3820.151011 shu

Analysis of ISG15-Modified Proteins from A549 Cells in Response to Influenza Virus Infection by Liquid Chromatography-Tandem Mass Spectrometry

1.
Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun 130062, China;
2.
Jilin Yatai Biological Pharmaceutical Inc, Changchun 130033, China;
3.
Central Laboratory, The Second Hospital of Jilin University, Changchun 130041, China

Received Date: 24 December 2015
Revised Date: 15 February 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21175055, 31372409), Jilin Province Science and Technology Department (Nos. 20110739, 20150204001YY), the Health and Family Planning Commission of Jilin Province (No.2015Z041) and Jilin University Bethune Project B (2012210)

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Interferon stimulated gene 15 kDa protein (ISG15) is the first ubiquitin-like protein identified, which plays vital roles in a variety of fields including viral infection and immunological regulation. In this study, liquid chromatography-tandem mass spectrometry was used to analyze ISG15-modified proteins in A549 cells in response to infection by influenza virus, which was enriched by immunoprecipitation. A total of 22 cellular host proteins were identified in A549 cells infected by influenza virus, including ubiquitin-like ISG15 protein, cyclin-T1, heat shock protein 71 kDa, caldesmon, eukaryotic translation initiation factor, and so on. Besides, non-structural protein (NS1) from influenza virus was also identified. Among the 22 host proteins identified, 6 proteins were also identified in the control non-infected A549 cells, including annexin A1, fructose-bisphosphate aldolase A, ATP synthase subunit g, enolase, actin, and tubulin. Bioinformatics analysis revealed that the identified ISG15-modified host proteins induced by influenza virus infection could be classified into 9 protein classes: chaperone, oxidoreductase, enzyme modulator, transferase, nucleic acid binding, transcription factor, kinase, cytoskeletal protein, and structural protein. This study provided a specific and effective tool for analyzing ISG15-modified proteins in proteome level.
- Influenza virus,
- Ubiquitin-like protein,
- Interferon stimulated gene 15 kDa protein,
- Liquid chromatography-tandem mass spectrometry
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
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沈阳化工大学材料科学与工程学院沈阳 110142