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Citation: WEI Ying, WAN Chuan-Ling, XUE Rong, LI Xiao-Jing, ZHANG Wen-Jun, PEI Feng-Kui. ¹H NMR-Based Metabonomic Study on Urine from Haematitum-Treated Rats[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(6): 857-963. doi: 10.11895/j.issn.0253-3820.150934 shu

¹H NMR-Based Metabonomic Study on Urine from Haematitum-Treated Rats

1.
Key Laboratory of Synthetic Rubber, Chinese Academy of Sciences, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
Department of Chemistry, Hebei University of Technology, Tianjin 300130, China

Received Date: 23 November 2015
Revised Date: 26 February 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 20975097, 21305134)

Figures(5)

Wistar rats were intragastrically administered with different doses (2, 5 and 10 g/kg body weight) of haematitum. ¹H NMR-based metabonomic analysis coupled with multivariate statistical analysis (principal component analysis and partial least squares-discriminant analysis) was used to analyze the metabolic profiles of the urine samples collected from the treated rats. Univariate analysis on the ¹H NMR spectra of urine (1 d before administration, 1-5 d post administration) was used to screen out the potential features of haematitum. Significant treatment related changes were observed for the levels of citrate, tuarine, creatinine, α-ketoglutarate, succinate and dimethylglycine, which could be used as potential features of haematitum. A trend of recovery in connection with dose levels was observed overtime. Such biochemical changes indicated that haematitum treatment at the dose of 2, 5 and 10 g/kg body weight affected the Krebs cycle and glucose metabolism, energy metabolism, choline metabolism and dimethylglycine metabolism in rats. These changes may attribute to the disturbances of hepatic function in 10 g/kg body weight group.
- Nuclear magnetic resonance,
- Metabonomics,
- Principal component analysis,
- Partial least squares-discriminant analysis,
- Haematitum
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