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Citation: QI Kai, TANG Xiao-Yan, WANG Min, ZHENG Xin, YANG Meng-Rui, ZHOU Jian. Analysis of Ribavirin and Its Metabolites in Fresh Eggs by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(6): 923-928. doi: 10.11895/j.issn.0253-3820.150925 shu

Analysis of Ribavirin and Its Metabolites in Fresh Eggs by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

QI Kai ^1,2, ,
TANG Xiao-Yan ¹ ,
WANG Min ¹ ,
ZHENG Xin ^1,2 ,
YANG Meng-Rui ¹ ,
ZHOU Jian ¹

1.
Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Agro-products, Chinese Academy of Agricultural & Science, Beijing 100081, China;
2.
National Centre of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China

Received Date: 17 November 2015
Revised Date: 12 January 2016

Fund Project: This work was supported by the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (No.2012BAD28B03).

Figures(3)

A rapid and convenient ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method for analysis of ribavirin and its two main kinds of metabolites, 1,2,4-triazole-3-carboxamide (TCONH₂) and 1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxylic acid (RTCOOH), in fresh eggs has been developed and validated. The sample was extracted with acetonitrile water (9:1, V/V), added N-hexane to move liposoluble substances, after centrifugation, the upper solvent was cleaned-up by C₁₈ and GCB, then determined by UPLC-MS/MS coupled with Agilent ZORBAX SB-Aq column (100 mm×3.0 mm, 1.8 μm). Over the concentration range of 2.0-200.0 μg/L (0.5-200.0 μg/L and 5.0-200.0 μg/L) for ribavirin (TCONH₂ and RTCOOH) in fresh egg matrix, the correlation coefficients (R²) of the calibration curves were above 0.99, and the limits of detection (LODs) were 0.54 μg/L (0.09 and 1.54 μg/L). The limits of quantitation (LOQs) were 1.79, 0.31 and 5.13 μg/L, respectively. At three spiked concentration levels of 5.0, 10.0 and 50.0 μg/L, the recoveries of ribavirin and RTCOOH ranged from 96.1% to 99.6% and 42.9% to 58.3%. And at three spiked concentration levels of 0.5, 2.0 and 5.0 μg/L, the recoveries of TCONH₂ ranged from 75.9% to 106.7%. The results of the determination in various real samples showed that the method is simple, accurate, rapid and suitable determination of ribavirin and its two main kinds of metabolites in fresh eggs.
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