Citation: LI Jing, LIU Xiao-Lei, ZHAO Xu, LI Wei, DAI Yu-Lin, YUE Hao. A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 451-455. doi: 10.11895/j.issn.0253-3820.150872 shu

A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry

LI Jing ¹ ,
LIU Xiao-Lei ² ,
ZHAO Xu ³ ,
LI Wei ⁴ ,
DAI Yu-Lin ^2,, ,
YUE Hao ^2,,

1.
¹ Hospital Affiliated to Changchun University of Traditional Chinese Medicine, Changchun 130021, China;
2.
² JiLin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China;
3.
³ The First Hosital of Jilin University, Changchun 130021, China;
4.
⁴ Shenzhen Third People's Hospital, Shenzhen 518112, China

Corresponding author: DAI Yu-Lin, YUE Hao,
Received Date: 1 November 2015
Available Online: 23 November 2015
Fund Project: 本文系公益性行业(农业)专项(No.20130311106)资助 (农业)专项(No.20130311106)

In this study, the rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry (RRLC-QTOF/MS) was used to profile the metabolites of urine samples from Childhood Pneumonia (CP) patients and healthy controls and find the potential biomarkers which can support evidence to early diagnose and cure the disease. Choose 10 CP patients (age 47.72±2.35 months) and 10 healthy controls (age 46.65±1.97 months). The urine samples were analyzed by RRLC-QTOF/MS and then the resulting data matrices were analyzed by principal components analysis (PCA) to find the potential biomarkers. Urine samples of CP patients were successfully distinguished from those of healthy controls. A total of two significantly changed metabolites have been found and identified as potential biomarkers. It is suggested that the disorder of purine metabolism and amino acid metabolism may play an important role in the mechanism of CP.
- Childhood Pneumonia,
- Rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry,
- Uric acid,
- Metabonomics
1. [1]
  1 Ngeow Y F, Suwanjutha S, Chantarojanasriri T, Wang F, Saniel M, Alejandria M, Hsueh P R, Ping Ing L, Seung Chul P, Jang Wook S, Aziah A, Youning L, Wing Hong S, Cecilia C L N, Hadiarto M, Hood A, Yuet Meng C. J. International Journal of Infectious Diseases, 2005, 9(3): 144-153
2. [2]
  2 Rudan I, Boschi-Pinto C, Biloglav Z, Mulholland K, Campbel H. J. Bulletin of the World Health Organization, 2008, 86(5): 408-416B
3. [3]
  3 Simon L, Gauvin F, Amre D K, Louis P S, Lacroix J. J.Clinical Infectious Diseases, 2004, 39(2): 206-217
4. [4]
  4 Toikka P, Irjala K, Juven T, Virkki R, Mertsola J, Leinonen M, Ruuskanen O. J. The Pediatric Infectious Disease Journal, 2000, 19(7): 598-602
5. [5]
  5 Nicholson J K. J. Molecular Systems Biology, 2006, 2(1): 52
6. [6]
  6 Holmes E, Tang H, Wang Y, Wang Y, Seger C. J. Plant Med., 2006, 72(9): 771-785
7. [7]
  7 Nicholson J K, Wilson I D. J. Nat Rev. Drug Discov., 2003, 2(8): 668-676
8. [8]
  8 GU Jin-Ning, NIU Jun, PI Zi-Feng, YUE Hao, WU Sui-Sheng, LIU Shu-Ying. Chinese J. Anal. Chem., 2013, 41(3): 371-376谷金宁, 牛俊, 皮子凤, 越皓, 吴绥生, 刘淑莹. 分析化学, 2013, 41(3): 371-376
9. [9]
  9 Dhakal K, Batabyal S, Wright W, Youngtae K, Mohanty S.J. Light: Science & Applications, 2015, 4, e352; doi: 10.1038/lsa.2015.125
10. [10]
  10 PANG Bo, YUE Hao, WANG En-Peng, WEI Hai-Tao, DAI Yu-Lin, LIU Shu-Ying, WU Shui-Sheng. Chinese J. Anal. Chem., 2015, 43(11): 1766-1771庞博, 越皓, 王恩鹏, 尉海涛, 戴雨霖, 刘淑莹, 吴绥生. 分析化学, 2015, 43(11): 1766-1771
11. [11]
  11 CHEN Yan-Hua, ZHANG Rui-Ping, SONG Yong-Mei, DONG Li-Jia, ZHAN Qi-Min, Zeper Abliz. Chinese J. Anal. Chem., 2011, 39(2): 173-177陈艳华, 张瑞萍, 宋咏梅, 董立佳, 詹启敏, 再帕尔·阿不力孜. 分析化学, 2011, 39(2): 173-177
12. [12]
  12 NAN Xiang-Zhen, PAN Guo-Quan,YAN Chun-Xue, YE Jing, HAN Guo-Qiang. J. Modern practical medicine, 2009, 21(5): 498-498南向珍, 潘国权, 严纯雪, 叶璟, 韩国强. 现代实用医学, 2009, 21(5): 498-498
13. [13]
  13 Gasse P, Riteau N, Charron S, Girre S, Fick L, Pétrilli V, Tschopp J, Lagente V, Quesniaux F J, Ryffel B, Couillin I. American Journal of Respiratory & Critical Care Medicine, 2009, 179(10): 903-913
14. [14]
  14 YAN Ping. The 14^th National Pediatric Academic Conference. 2006. C567: 436-437颜萍. 中华医学会第十四次全国儿科学术会议论文汇编, 2006. C567: 436-437
15. [15]
  15 Ogihara T, Kim H S, Hirano K, Imanishi M, Ogihara H, Tamai H, Okamoto R, Mino M. J. Biology of the Neonate, 1998, 73(1): 24-33
16. [16]
  16 LI Fei, YAO Jun-Hu. J. Feed industry, 2009, 30(20): 47-50李飞, 姚军虎. 饲料工业, 2009, 30(20): 47-50
17. [17]
  17 Schicho R, Shaykhutdinov R, Ngo J, Nazyrova A, Schneider C, Panaccione R, Kaplan G G, Vogel H J, Storr M. J. Proteome Res., 2012, 11(6): 3344-3357
1. [1]
  Hao BAI , Weizhi JI , Jinyan CHEN , Hongji LI , Mingji LI . Preparation of Cu₂O/Cu-vertical graphene microelectrode and detection of uric acid/electroencephalogram. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1309-1319. doi: 10.11862/CJIC.20240001
2. [2]
  Hongyao Li , Youyan Liu , Luwei Dai , Min Yang , Qihui Wang . The Blessing of Indium Sulfide：Confronting the Narrow Path with Uric Acid. University Chemistry, 2024, 39(5): 325-335. doi: 10.3866/PKU.DXHX202311104
3. [3]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
4. [4]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
5. [5]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
6. [6]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
7. [7]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
8. [8]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
9. [9]
  Jia Huo , Jia Li , Yongjun Li , Yuzhi Wang . Ideological and Political Design of Physical Chemistry Teaching: Chemical Potential of Any Component in an Ideal-Dilute Solution. University Chemistry, 2024, 39(2): 14-20. doi: 10.3866/PKU.DXHX202307075
10. [10]
  Hongwei Ma , Fang Zhang , Hui Ai , Niu Zhang , Shaochun Peng , Hui Li . Integrated Crystallographic Teaching with X-ray,TEM and STM. University Chemistry, 2024, 39(3): 5-17. doi: 10.3866/PKU.DXHX202308107
11. [11]
  Jiantao Zai , Hongjin Chen , Xiao Wei , Li Zhang , Li Ma , Xuefeng Qian . The Learning-Centered Problem-Oriented Experimental Teaching. University Chemistry, 2024, 39(4): 40-47. doi: 10.3866/PKU.DXHX202309023
12. [12]
  Yang Liu , Peng Chen , Lei Liu . Chemistry “101 Plan”: Design and Construction of Chemical Biology Textbook. University Chemistry, 2024, 39(10): 45-51. doi: 10.12461/PKU.DXHX202407085
13. [13]
  Weitai Wu , Laiying Zhang , Yuan Chun , Liang Qiao , Bin Ren . Course Design of Chemical Measurement Experiments in Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 64-68. doi: 10.12461/PKU.DXHX202409031
14. [14]
  Tianyu Feng , Guifang Jia , Peng Zou , Jun Huang , Zhanxia Lü , Zhen Gao , Chu Wang . Construction of the Chemistry Biology Experiment Course in the Chemistry “101 Program”. University Chemistry, 2024, 39(10): 69-77. doi: 10.12461/PKU.DXHX202409002
15. [15]
  Laiying Zhang , Weitai Wu , Yiru Wang , Shunliu Deng , Zhaobin Chen , Jiajia Chen , Bin Ren . Practices for Improving the Course of Chemical Measurement Experiments in the Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 107-112. doi: 10.12461/PKU.DXHX202409032
16. [16]
  Yuqiao Zhou , Weidi Cao , Shunxi Dong , Lili Lin , Xiaohua Liu . Study on the Teaching Reformation of Practical X-ray Crystallography. University Chemistry, 2024, 39(3): 23-28. doi: 10.3866/PKU.DXHX202303003
17. [17]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
18. [18]
  Yan Su , Xiuyun Wang , Huimin Guo , Yanjuan Zhang , Xinwen Zhang , Yunting Shang , Wenfeng Jiang . To Cultivate Scientific Literacy by Learning, Thinking, Practicing and Understanding, To Utilize the “Smart Eye” Expertise by Integrating of Knowledge and Action: Ideological and Political Construction of Analytical Chemistry Experiment Course. University Chemistry, 2024, 39(2): 196-202. doi: 10.3866/PKU.DXHX202308003
19. [19]
  Zhenming Xu , Mingbo Zheng , Zhenhui Liu , Duo Chen , Qingsheng Liu . Experimental Design of Project-Driven Teaching in Computational Materials Science: First-Principles Calculations of the LiFePO₄ Cathode Material for Lithium-Ion Batteries. University Chemistry, 2024, 39(4): 140-148. doi: 10.3866/PKU.DXHX202307022
20. [20]
  Qiying Xia , Guokui Liu , Yunzhi Li , Yaoyao Wei , Xia Leng , Guangli Zhou , Aixiang Wang , Congcong Mi , Dengxue Ma . Construction and Practice of “Teaching-Learning-Assessment Integration” Model Based on Outcome Orientation: Taking “Structural Chemistry” as an Example. University Chemistry, 2024, 39(10): 361-368. doi: 10.3866/PKU.DXHX202311007

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(316)
HTML views(24)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142