Citation: HAN Wan-Qing, WU Chu-Sen, WU Yu-Luan, DONG Hao, WANG Li, WANG Bin, XIAN Yan-Ping, LUO Hai-Ying. Analysis of 32 Kinds of β-Agonists, β-Blockers and Glycopeptide Antibiotics in Animal Meat by UPLC-MS/MS[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 289-296. doi: 10.11895/j.issn.0253-3820.150830 shu

Analysis of 32 Kinds of β-Agonists, β-Blockers and Glycopeptide Antibiotics in Animal Meat by UPLC-MS/MS

1.
Guangzhou Quality Supervision and Testing Institute, National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou Key Laboratory of Detection Technology for Food Safety, Guangzhou Research Centre of Risk Dynamic Detection and Early Warning for Food Safety, Guangzhou 510000, China

Corresponding author: LUO Hai-Ying,
Received Date: 20 October 2015
Available Online: 13 November 2015
Fund Project: 本文系国家科技部港澳台科技合作专项基金资助(No. 2013DFH30070) (No. 2013DFH30070)

A high-throughput method was established for the simultaneous determination of 27 kinds of β-agonists, 3 kinds of β-blockers and 2 kinds of glycopeptide antibiotics in animal meat by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The sample underwent enzymolysis and the protein precipitation treatment was extracted with a mixture of ethyl acetate-isopropanol (6:4, V/V), and cleaned up at a Strata-X-C solid phase extraction cartridge. The 32 targets were separated on a Waters BEH C₁₈ column by gradient elution with acetonitrile-0.1% formaic acid as mobile phase, ionized with positive electrospray ionization (ESI+), detected under multiple reaction monitoring mode, and quantified with external standard method. The results showed that the target compounds displayed excellent linearity in the concentration of 5-500 μg/L with correlation coefficients larger than 0.995. The limits of detection were 5 μg/kg for dopamine, reproterol, vancomycin and norvancomycin, and were 3 μg/kg for the others. The average recoveries were in the range of 83.6%-103.0% at two spiked levels. The intra-day RSDs were ranged from 3.9% to 10.4%, and the inter-day RSDs were no more than 9.8%. The developed method was accurate and sensitive, and was suitable for the high-throughput quantitative and qualitative analysis of β-agonists, β-blockers and glycopeptide antibiotics in animal meat.
- β-Agonist,
- β-Blocker,
- Glycopeptide antibiotic,
- Animal meat,
- Ultra-high performance liquid chromatography-tandem mass spectrometry
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