Citation: YANG Li-Zhu, ZHU Jing, ZHANG Ren-Yi, MA Dan-Qi, HE Pin-Gang, FANG Yu-Zhi. DNA Biosensor Based on Large Area of Gold Particles on Aligned Carbon Nanotubes for Detection of Promyelocytic Leukaemia/Retinoic Acid Receptor α Fusion Gene[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 391-395. doi: 10.11895/j.issn.0253-3820.150773 shu

DNA Biosensor Based on Large Area of Gold Particles on Aligned Carbon Nanotubes for Detection of Promyelocytic Leukaemia/Retinoic Acid Receptor α Fusion Gene

YANG Li-Zhu ^1,, ,
ZHU Jing ² ,
ZHANG Ren-Yi ² ,
MA Dan-Qi ^1,3 ,
HE Pin-Gang ² ,
FANG Yu-Zhi ²

1.
¹ School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China;
2.
² Department of Chemistry, East China Normal University, Shanghai 200241, China;
3.
³ Family Planning Publicity & Education Technical Advising Center of Luqiao, Taizhou 318050, China

Corresponding author: YANG Li-Zhu,
Received Date: 1 October 2015
Available Online: 5 January 2016
Fund Project: 本文系国家自然科学基金项目(No.31300819) (No.31300819)浙江省自然科学基金项目(No.LQ12B05004)资助 (No.LQ12B05004)

A novel electrochemical DNA biosensor was fabricated based on the large area of gold particles on the aligned carbon nanotubes for the detection of promyelocytic leukaemia/retinoic acid receptor α (PML/RARα) fusion gene in acute promyelocytic leukemia. Firstly, the thiol-modified single-stranded DNA (ssDNA, probe 1) was immobilized on the gold particles sputtered on the aligned carbon nanotubes electrode by self-assembly technique. Then, amino-modified ssDNA and carboxyl-modified CdTe quantum dots (QDs) were combined to get CdTe-modified DNA probe through an amidization reaction. After hybridization with target DNA, a sandwich-type assay structure was formed. Finally, the CdTe quantum dots captured on the electrode surface were detected by differential pulse anodic stripping voltammetry. The change of peak current of Cd²⁺ on the electrode was found to be linear with the logarithm of target DNA concentration in the range of 1.0×10^-12 mol/L to 1.0×10^-8 mol/L. The Linear equation was i_pa (μA)=1.626+0.132lgC (mol/L) (the correlation coefficient R was 0.996) and the detection limit was 4.0×10^-13 mol/L (3σ). The fabricated DNA biosensor exhibited excellent reproducibility and stability.
- Aligned carbon nanotubes,
- Gold particles,
- Acute promyelocytic leukemia,
- DNA biosensor,
- Differential pulse anodic stripping voltammetry
1. [1]
  1 Bennett J, Catovsky D, Daniel M, Flandrin G, Galton D A G, Gralnick H R, Sultan C. Br. J. Haematol., 1976, 33(4): 451-458
2. [2]
  2 Han J Y, Kim K E, Kim K H, Park J I, Kim J S. Leukemia Res., 2007, 31(2): 239-243
3. [3]
  3 Mokany E, Todd A V, Fuery C J, Applegate T L. Methods Mol. Med., 2006, 125: 127-147
4. [4]
  4 ZHANG Qiang, LI Yu-Yun, XU Xiu-Cai, ZHAI Zhi-Min. Chinese J. Clin. Lab. Sci., 2010, 28(4): 281-282张强, 李玉云, 徐修才, 翟志敏. 临床检验杂志, 2010, 28(4): 281-282
5. [5]
  5 Sun W, Zhang Y Y, Hu A H, Lu Y X, Shi F, Lei B X, Sun Z F. Electroanalysis, 2013, 25(6): 1417-1424
6. [6]
  6 CHENG Gui-Fang, HUANG Cui-Hua, ZHAO Jie, TAN Xue-Lian, HE Pin-Gang, FANG Yu-Zhi. Chinese J. Anal. Chem., 2009, 37(2): 169-173程圭芳, 黄翠华, 赵洁, 谭雪莲, 何品刚, 方禹之. 分析化学, 2009, 37(2): 169-173
7. [7]
  7 Yola M L, Eren T, Atar, N. Electrochim. Acta, 2014, 125(10): 38-47
8. [8]
  8 Li Y, Deng J, Fang L C, Yu K K, Huang H, L L, Liang W B, Zheng J S. Biosen. Bioelectron., 2015, 63(15): 1-6
9. [9]
  9 XU Kai, YE Zun-Zhong, YING Yi-Bin. Chinese J. Anal. Chem., 2008, 36(8): 1113-1116许凯, 叶尊忠, 应义斌. 分析化学, 2008, 36(8): 1113-1116
10. [10]
  10 Jin L, Bower C, Zhou O. Appl. Phys. Lett., 1998, 73(9): 1197-1199
11. [11]
  11 Vigolo B, Pénicaud A, Coulon C, Sauder C, Pailler R, Journet C, Bernier P, Poulin P. Science, 2000, 290(5495): 1331-1334
12. [12]
  12 Wang S G, Wang R, Sellin P J, Zhang Q. Biochem. Biophys. Res. Commun., 2004, 325(4): 1433-1437
13. [13]
  13 Yang J, Xu Y, He P G,Fang Y Z. Electroanalysis., 2013, 25(10): 2345-2353
14. [14]
  14 Yang L Z, Xu Y, Wang X H, Zhu J, Zhang R Y, He P G, Fang Y Z. Anal. Chim. Acta, 2011, 689(1): 39-46
15. [15]
  15 SONG Hai-Yan, LI Yan-Nan, ZHAO Kun, YE Xiao-Yan, HE Pin-Gang, SUN Zhuo, FANG Yu-Zhi. Chem. J. Chinese Universities, 2007, 28(9): 1622-1626宋海燕, 李艳楠, 赵琨, 叶晓燕, 何品刚, 孙卓, 方禹之. 高等学校化学学报, 2007, 28(9): 1622-1626
16. [16]
  16 Chen X, Wang Y, Zhou J, Yan W, Li X, Zhu J J. Anal. Chem., 2008, 80(6): 2133-2140
17. [17]
  17 Angerstein-Kozlowska H, Conway B E, Hamelin A, Stoicovicu L. J. Electroanal. Chem. Interf. Electrochem., 1987, 228(1-2): 429-453
18. [18]
  18 Fan H, Xing R, Wang X H, Xu Y, Wang Q J, He PG, Fang Y Z. Electroanalysis., 2010, 22(15): 1781-1786
1. [1]
  Meiqing Yang , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-. doi: 10.3866/PKU.WHXB202310046
2. [2]
  Xiufang Wang , Donglin Zhao , Kehua Zhang , Xiaojie Song . “Preparation of Carbon Nanotube/SnS₂ Photoanode Materials”: A Comprehensive University Chemistry Experiment. University Chemistry, 2024, 39(4): 157-162. doi: 10.3866/PKU.DXHX202308025
3. [3]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
4. [4]
  Kun Li , Na Gao , Shuangyan Huan , Yuzhi Wang . Design of Ideological and Political Education for the Experiment of Detecting Cadmium with Anodic Stripping Voltammetry. University Chemistry, 2024, 39(2): 155-161. doi: 10.3866/PKU.DXHX202307068
5. [5]
  Hailang JIA , Hongcheng LI , Pengcheng JI , Yang TENG , Mingyun GUAN . Preparation and performance of N-doped carbon nanotubes composite Co₃O₄ as oxygen reduction reaction electrocatalysts. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 693-700. doi: 10.11862/CJIC.20230402
6. [6]
  Qiaoqiao BAI , Anqi ZHOU , Xiaowei LI , Tang LIU , Song LIU . Construction of pressure-temperature dual-functional flexible sensors and applications in biomedicine. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2259-2274. doi: 10.11862/CJIC.20240128
7. [7]
  Yongming Guo , Jie Li , Chaoyong Liu . Green Improvement and Educational Design in the Synthesis and Characterization of Silver Nanoparticles. University Chemistry, 2024, 39(3): 258-265. doi: 10.3866/PKU.DXHX202309057
8. [8]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
9. [9]
  Haihua Yang , Minjie Zhou , Binhong He , Wenyuan Xu , Bing Chen , Enxiang Liang . Synthesis and Electrocatalytic Performance of Iron Phosphide@Carbon Nanotubes as Cathode Material for Zinc-Air Battery: a Comprehensive Undergraduate Chemical Experiment. University Chemistry, 2024, 39(10): 426-432. doi: 10.12461/PKU.DXHX202405100
10. [10]
  Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . 倍增型全聚合物光电探测器及其在光电容积描记传感器上的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2311021-. doi: 10.3866/PKU.WHXB202311021
11. [11]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
12. [12]
  Zhaoxin LI , Ruibo WEI , Min ZHANG , Zefeng WANG , Jing ZHENG , Jianbo LIU . Advancements in the construction of inorganic protocells and their cell mimic and bio-catalytical applications. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2286-2302. doi: 10.11862/CJIC.20240235
13. [13]
  Zian Fang , Qianqian Wen , Yidi Wang , Hongxia Ouyang , Qi Wang , Qiuping Li . The Test Paper for Metal Ion: A Popular Science Experiment Based on Color Aesthetics. University Chemistry, 2024, 39(5): 108-115. doi: 10.3866/PKU.DXHX202310032
14. [14]
  Donghui PAN , Yuping XU , Xinyu WANG , Lizhen WANG , Junjie YAN , Dongjian SHI , Min YANG , Mingqing CHEN . Preparation and in vivo tracing of ⁶⁸Ga-labeled PM_2.5 mimetic particles for positron emission tomography imaging. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 669-676. doi: 10.11862/CJIC.20230468
15. [15]
  Jinghan ZHANG , Guanying CHEN . Progress in the application of rare-earth-doped upconversion nanoprobes in biological detection. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2335-2355. doi: 10.11862/CJIC.20240249
16. [16]
  Hong RAO , Yang HU , Yicong MA , Chunxin LÜ , Wei ZHONG , Lihua DU . Synthesis and in vitro anticancer activity of phenanthroline-functionalized nitrogen heterocyclic carbene homo- and heterobimetallic silver/gold complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2429-2437. doi: 10.11862/CJIC.20240275
17. [17]
  Mengfei He , Chao Chen , Yue Tang , Si Meng , Zunfa Wang , Liyu Wang , Jiabao Xing , Xinyu Zhang , Jiahui Huang , Jiangbo Lu , Hongmei Jing , Xiangyu Liu , Hua Xu . Epitaxial Growth of Nonlayered 2D MnTe Nanosheets with Thickness-Tunable Conduction for p-Type Field Effect Transistor and Superior Contact Electrode. Acta Physico-Chimica Sinica, 2025, 41(2): 100016-. doi: 10.3866/PKU.WHXB202310029
18. [18]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
19. [19]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036
20. [20]
  Chenye An , Abiduweili Sikandaier , Xue Guo , Yukun Zhu , Hua Tang , Dongjiang Yang . 红磷纳米颗粒嵌入花状CeO₂分级S型异质结高效光催化产氢. Acta Physico-Chimica Sinica, 2024, 40(11): 2405019-. doi: 10.3866/PKU.WHXB202405019

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(292)
HTML views(18)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142