Citation: MEI Xiao-Qi, HE Xiu-Ping, WANG Jiang-Tao. Separation and Determination of Gonyautoxins 1,4 in Seawater by Molecularly Imprinted Solid Phase Extraction Coupled with High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 212-217. doi: 10.11895/j.issn.0253-3820.150744 shu

Separation and Determination of Gonyautoxins 1,4 in Seawater by Molecularly Imprinted Solid Phase Extraction Coupled with High Performance Liquid Chromatography

1.
Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education, Ocean University of China, Qingdao 266100, China

Corresponding author: WANG Jiang-Tao,
Received Date: 19 September 2015
Available Online: 4 December 2015

A kind of molecular imprinted polymer (MIP) was synthesized by bulk polymerization using guanosine as dummy template molecule. The results of Fourier transform infrared spectrometer (FT-IR) and scanning electron microscope showed that MIP had homogenous and uniform-sized cavities. It was confirmed that MIP had higher binding affinity and selectivity towards gonyautoxins 1,4 (GTX1,4) than NIP according to the static equilibrium adsorption. The solid phase extraction columns were packed with MIP. The maximum recovery (85.05%) of MISPE was achieved using 0.1 mol/L acetic acid as washing solution and ethanol-water (95:5, V/V) as elution solution. Then the concentrations of GTX1,4 from culture solution of Alexandrium minutum and Alexandrium tamarense were determined to be 1.10 and 0.99 μg/L, respectively, under the optimum conditions of washing and elution, and RSD was 3.3% and 4.4%, respectively, indicated good LOD and higher precision.
- Gonyautoxins 1,4,
- Molecularly imprinted solid phase extraction,
- Separation and preconcentration,
- Alexandrium minutum,
- Alexandrium tamarense
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