Citation: MEI Xiao-Qi, HE Xiu-Ping, WANG Jiang-Tao. Separation and Determination of Gonyautoxins 1,4 in Seawater by Molecularly Imprinted Solid Phase Extraction Coupled with High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 212-217. doi: 10.11895/j.issn.0253-3820.150744 shu

Separation and Determination of Gonyautoxins 1,4 in Seawater by Molecularly Imprinted Solid Phase Extraction Coupled with High Performance Liquid Chromatography

1.
Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education, Ocean University of China, Qingdao 266100, China

Corresponding author: WANG Jiang-Tao,
Received Date: 19 September 2015
Available Online: 4 December 2015

A kind of molecular imprinted polymer (MIP) was synthesized by bulk polymerization using guanosine as dummy template molecule. The results of Fourier transform infrared spectrometer (FT-IR) and scanning electron microscope showed that MIP had homogenous and uniform-sized cavities. It was confirmed that MIP had higher binding affinity and selectivity towards gonyautoxins 1,4 (GTX1,4) than NIP according to the static equilibrium adsorption. The solid phase extraction columns were packed with MIP. The maximum recovery (85.05%) of MISPE was achieved using 0.1 mol/L acetic acid as washing solution and ethanol-water (95:5, V/V) as elution solution. Then the concentrations of GTX1,4 from culture solution of Alexandrium minutum and Alexandrium tamarense were determined to be 1.10 and 0.99 μg/L, respectively, under the optimum conditions of washing and elution, and RSD was 3.3% and 4.4%, respectively, indicated good LOD and higher precision.
- Gonyautoxins 1,4,
- Molecularly imprinted solid phase extraction,
- Separation and preconcentration,
- Alexandrium minutum,
- Alexandrium tamarense
1. [1]
  1 ZHOU Ming-Jiang,LI Jun. Chinese Journal of Marine Drugs, 1999, 18(3): 48-54 周名江, 李钧. 中国海洋药物, 1999, 18(3): 48-54
2. [2]
  2 Asakawa M, Miyazawa K, Takayama H, Noguchi T. Toxicon, 1995, 33(5): 691-697
3. [3]
  3 Lim P T, Ogata T. Toxicon, 2005, 45(6): 699-710
4. [4]
  4 Vale P, Rangel I, Silva B, Coelho P,Vilar A. Toxicon, 2009, 53(1): 176-183
5. [5]
  5 Hakanen P, Suikkanen S, Franzén J, Franzén H, Kankaanpää H, Kremp A. Harmful Algae, 2012, 15: 91-99
6. [6]
  6 Zhou M, Li J,Luckas B, Yu R, Yan T, Hummert C, Kastrup S. Marine Pollut. Bull., 1999, 39(1): 331-334
7. [7]
  7 de Carvalho M, Jacinto J, Ramos N, Oliveira V, Melo T, Sá J. J. Neurol., 1998, 245(8): 551-554
8. [8]
  8 Benton B J, Keller S A,Spriggs D L, Capacio B R, Chang F C T. Toxicon, 1998, 36(4): 571-588
9. [9]
  9 HU Hao-Yan, TANG Jing-Liang, WANG Yi-Ming, HUANG Bei, CHEN Yi-Hua, MAO Hong-Yue. Environmental Pollution and Control, 2006, 27(6): 470-472 胡颢琰, 唐静亮, 王益鸣, 黄备, 陈逸华, 毛宏跃. 环境污染与防治, 2006, 27(6): 470-472
10. [10]
  10 Velez P, Sierralta J, Alcayaga C, Fonseca M, Loyola H, Johns D C, Tomaselli G F, Marbán E, Suárez-Isla B A. Toxicon, 2001, 39(7): 929-935
11. [11]
  11 ZHANGWen, WANG Jun, SU Yong-Quan. Marine Sciences, 2005: 6 张纹, 王军, 苏永全. 海洋科学, 2005: 6
12. [12]
  12 Usleber E, Dietrich R, Bürk C, Schneider E, Martlbäuer E. J. AOAC Inter., 2001, 84(5): 1649-1656
13. [13]
  13 SUN Xing-Quan, ZHENG Qiu-Yue, PANG Yan-Hua, LI Yi-Chen, XIAO Shan-Shan, XU Jing, CAO Ji-Juan. Chinese J. Anal. Chem., 2013, 41(9): 1423-1427 孙兴权, 郑秋月, 庞艳华, 李一尘, 肖珊珊, 徐静, 曹际娟. 分析化学, 2013, 41(9): 1423-1427
14. [14]
  14 Lawrence J F, Niedzwiadek B, Menard C. J. AOAC Inter., 2005, 88(6): 1714-1732
15. [15]
  15 Oshima Y. J. AOAC Inter., 1995, 78(2): 528-532
16. [16]
  16 ZHANGShao-wen, GAO Ya-Hui. Chinese Journal of Analysis Laboratory, 2012, 31(9): 106-109 张少文, 高亚辉. 分析实验室, 2012, 31(9): 106-109
17. [17]
  17 LIANZi-Ru, PhD Thesis, Ocean University of China, 2013 连子如. 博士论文, 中国海洋大学, 2013
18. [18]
  18 Rodríguez P, Alfonso A, Botana A M, Vieytes M R, Botana L M. Toxicon, 2010, 56(3): 448-457
19. [19]
  19 Lian Z R, Wang J T. Environ. Pollut., 2013, 182: 385-391
1. [1]
  Xinyu Liu , Weiran Hu , Zhengkai Li , Wei Ji , Xiao Ni . Algin Lab: Surging Luminescent Sea. University Chemistry, 2024, 39(5): 396-404. doi: 10.3866/PKU.DXHX202312021
2. [2]
  Junjie Zhang , Yue Wang , Qiuhan Wu , Ruquan Shen , Han Liu , Xinhua Duan . Preparation and Selective Separation of Lightweight Magnetic Molecularly Imprinted Polymers for Trace Tetracycline Detection in Milk. University Chemistry, 2024, 39(5): 251-257. doi: 10.3866/PKU.DXHX202311084
3. [3]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
4. [4]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
5. [5]
  Rong Lai , Jie Li , Xianfang Xu , Shui Hu , Tao Chen , Houjin Li , Guping Hu , Hongyan Chen , Fang Zhu . Taking the Overall Relocation as an Opportunity to Accelerate the Development of Chemical Experimental Teaching Centers: A Case Study of the National Demonstration Center for Experimental Chemistry Education at Sun Yat-Sen University. University Chemistry, 2024, 39(4): 33-39. doi: 10.3866/PKU.DXHX202310115
6. [6]
  Jie Li , Rong Lai , Xianfang Xu , Houjin Li , Hongyan Chen , Fang Zhu . Exploration and Practice in the Construction of the National Demonstration Center for Experimental Chemistry Education: A Case Study of Sun Yat-sen University. University Chemistry, 2024, 39(7): 223-229. doi: 10.12461/PKU.DXHX202406054
7. [7]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
8. [8]
  Peipei Sun , Jinyuan Zhang , Yanhua Song , Zhao Mo , Zhigang Chen , Hui Xu . 引入内建电场增强光载流子分离以促进H₂的生产. Acta Physico-Chimica Sinica, 2024, 40(11): 2311001-. doi: 10.3866/PKU.WHXB202311001
9. [9]
  Lan Ma , Cailu He , Ziqi Liu , Yaohan Yang , Qingxia Ming , Xue Luo , Tianfeng He , Liyun Zhang . Magical Surface Chemistry: Fabrication and Application of Oil-Water Separation Membranes. University Chemistry, 2024, 39(5): 218-227. doi: 10.3866/PKU.DXHX202311046
10. [10]
  Wendian XIE , Yuehua LONG , Jianyang XIE , Liqun XING , Shixiong SHE , Yan YANG , Zhihao HUANG . Preparation and ion separation performance of oligoether chains enriched covalent organic framework membrane. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1528-1536. doi: 10.11862/CJIC.20240050
11. [11]
  Xinhao Yan , Guoliang Hu , Ruixi Chen , Hongyu Liu , Qizhi Yao , Jiao Li , Lingling Li . Polyethylene Glycol-Ammonium Sulfate-Nitroso R Salt System for the Separation of Cobalt (II). University Chemistry, 2024, 39(6): 287-294. doi: 10.3866/PKU.DXHX202310073
12. [12]
  Jie ZHAO , Sen LIU , Qikang YIN , Xiaoqing LU , Zhaojie WANG . Theoretical calculation of selective adsorption and separation of CO₂ by alkali metal modified naphthalene/naphthalenediyne. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 515-522. doi: 10.11862/CJIC.20230385
13. [13]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
14. [14]
  Shui Hu , Houjin Li , Zhenming Zang , Lianyun Li , Rong Lai . Integration of Science and Education Promotes the Construction of Undergraduate-to-Master’s Integration Experimental Courses: A Case Study on the Extraction, Separation and Identification of Artemisinin from Artemisia annua. University Chemistry, 2024, 39(4): 314-321. doi: 10.3866/PKU.DXHX202310063
15. [15]
  Yang Chen , Peng Chen , Yuyang Song , Yuxue Jin , Song Wu . Application of Chemical Transformation Driven Impurity Separation in Experiments Teaching: A Novel Method for Purification of α-Fluorinated Mandelic Acid. University Chemistry, 2024, 39(6): 253-263. doi: 10.3866/PKU.DXHX202310077
16. [16]
  Lihui Jiang , Wanrong Dong , Hua Yang , Yongqing Xia , Hongjian Peng , Jun Yuan , Xiaoqian Hu , Zihan Zeng , Yingping Zou , Yiming Luo . Study on Extraction of p-Hydroxyacetophenone. University Chemistry, 2024, 39(11): 259-268. doi: 10.12461/PKU.DXHX202402056
17. [17]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
18. [18]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
19. [19]
  Changjun You , Chunchun Wang , Mingjie Cai , Yanping Liu , Baikang Zhu , Shijie Li . 引入内建电场强化BiOBr/C₃N₅ S型异质结中光载流子分离以实现高效催化降解微污染物. Acta Physico-Chimica Sinica, 2024, 40(11): 2407014-. doi: 10.3866/PKU.WHXB202407014
20. [20]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(396)
HTML views(50)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142