Citation: MEI Xiao-Qi, HE Xiu-Ping, WANG Jiang-Tao. Separation and Determination of Gonyautoxins 1,4 in Seawater by Molecularly Imprinted Solid Phase Extraction Coupled with High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 212-217. doi: 10.11895/j.issn.0253-3820.150744 shu

Separation and Determination of Gonyautoxins 1,4 in Seawater by Molecularly Imprinted Solid Phase Extraction Coupled with High Performance Liquid Chromatography

1.
Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education, Ocean University of China, Qingdao 266100, China

Corresponding author: WANG Jiang-Tao,
Received Date: 19 September 2015
Available Online: 4 December 2015

A kind of molecular imprinted polymer (MIP) was synthesized by bulk polymerization using guanosine as dummy template molecule. The results of Fourier transform infrared spectrometer (FT-IR) and scanning electron microscope showed that MIP had homogenous and uniform-sized cavities. It was confirmed that MIP had higher binding affinity and selectivity towards gonyautoxins 1,4 (GTX1,4) than NIP according to the static equilibrium adsorption. The solid phase extraction columns were packed with MIP. The maximum recovery (85.05%) of MISPE was achieved using 0.1 mol/L acetic acid as washing solution and ethanol-water (95:5, V/V) as elution solution. Then the concentrations of GTX1,4 from culture solution of Alexandrium minutum and Alexandrium tamarense were determined to be 1.10 and 0.99 μg/L, respectively, under the optimum conditions of washing and elution, and RSD was 3.3% and 4.4%, respectively, indicated good LOD and higher precision.
- Gonyautoxins 1,4,
- Molecularly imprinted solid phase extraction,
- Separation and preconcentration,
- Alexandrium minutum,
- Alexandrium tamarense
1. [1]
  1 ZHOU Ming-Jiang,LI Jun. Chinese Journal of Marine Drugs, 1999, 18(3): 48-54 周名江, 李钧. 中国海洋药物, 1999, 18(3): 48-54
2. [2]
  2 Asakawa M, Miyazawa K, Takayama H, Noguchi T. Toxicon, 1995, 33(5): 691-697
3. [3]
  3 Lim P T, Ogata T. Toxicon, 2005, 45(6): 699-710
4. [4]
  4 Vale P, Rangel I, Silva B, Coelho P,Vilar A. Toxicon, 2009, 53(1): 176-183
5. [5]
  5 Hakanen P, Suikkanen S, Franzén J, Franzén H, Kankaanpää H, Kremp A. Harmful Algae, 2012, 15: 91-99
6. [6]
  6 Zhou M, Li J,Luckas B, Yu R, Yan T, Hummert C, Kastrup S. Marine Pollut. Bull., 1999, 39(1): 331-334
7. [7]
  7 de Carvalho M, Jacinto J, Ramos N, Oliveira V, Melo T, Sá J. J. Neurol., 1998, 245(8): 551-554
8. [8]
  8 Benton B J, Keller S A,Spriggs D L, Capacio B R, Chang F C T. Toxicon, 1998, 36(4): 571-588
9. [9]
  9 HU Hao-Yan, TANG Jing-Liang, WANG Yi-Ming, HUANG Bei, CHEN Yi-Hua, MAO Hong-Yue. Environmental Pollution and Control, 2006, 27(6): 470-472 胡颢琰, 唐静亮, 王益鸣, 黄备, 陈逸华, 毛宏跃. 环境污染与防治, 2006, 27(6): 470-472
10. [10]
  10 Velez P, Sierralta J, Alcayaga C, Fonseca M, Loyola H, Johns D C, Tomaselli G F, Marbán E, Suárez-Isla B A. Toxicon, 2001, 39(7): 929-935
11. [11]
  11 ZHANGWen, WANG Jun, SU Yong-Quan. Marine Sciences, 2005: 6 张纹, 王军, 苏永全. 海洋科学, 2005: 6
12. [12]
  12 Usleber E, Dietrich R, Bürk C, Schneider E, Martlbäuer E. J. AOAC Inter., 2001, 84(5): 1649-1656
13. [13]
  13 SUN Xing-Quan, ZHENG Qiu-Yue, PANG Yan-Hua, LI Yi-Chen, XIAO Shan-Shan, XU Jing, CAO Ji-Juan. Chinese J. Anal. Chem., 2013, 41(9): 1423-1427 孙兴权, 郑秋月, 庞艳华, 李一尘, 肖珊珊, 徐静, 曹际娟. 分析化学, 2013, 41(9): 1423-1427
14. [14]
  14 Lawrence J F, Niedzwiadek B, Menard C. J. AOAC Inter., 2005, 88(6): 1714-1732
15. [15]
  15 Oshima Y. J. AOAC Inter., 1995, 78(2): 528-532
16. [16]
  16 ZHANGShao-wen, GAO Ya-Hui. Chinese Journal of Analysis Laboratory, 2012, 31(9): 106-109 张少文, 高亚辉. 分析实验室, 2012, 31(9): 106-109
17. [17]
  17 LIANZi-Ru, PhD Thesis, Ocean University of China, 2013 连子如. 博士论文, 中国海洋大学, 2013
18. [18]
  18 Rodríguez P, Alfonso A, Botana A M, Vieytes M R, Botana L M. Toxicon, 2010, 56(3): 448-457
19. [19]
  19 Lian Z R, Wang J T. Environ. Pollut., 2013, 182: 385-391
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